Yasuda-Yasaki Y, Yoshida T O
Scand J Immunol. 1975;4(4):357-67. doi: 10.1111/j.1365-3083.1975.tb02637.x.
An antigenic substance reactive with autoantibodies found in patients with cancer and autoimmune diseases was isolated from calf thymus. The purification procedure included extraction of the tissues with acetone powder, batch and column chromatography on DEAE-resins, ammonium sulfate precipitation, gel filtration on Sephadex G-200, and affinity chromatography on antibody-Sepharose 4B. Indirect immunofluorescence examination of cultured human embryo cells, using the serum of patients with nasopharyngeal cancer, showed a speckled nuclear pattern. The antigenic factor was a soluble acidic protein with a pI of 5.0 and a molecular weight of 250,000. The antigenic activities of this purified substance from calf thymus, and of the material on the cultured human embryo cells, were destroyed by proteases, ribonuclease, and alkaline phosphatase. The determinants were also sensitive to periodate oxidation. Thermal stability to 60 degree C and pH stability between 2.6 and 8.5 were demonstrated. Cross-reactivity of the antigenic substance with antibodies isolated from individuals with cancer and autoimmune diseases was shown by immunofluorescence, with appropriate blocking and absorption controls.
从牛胸腺中分离出一种与癌症患者和自身免疫性疾病患者体内自身抗体发生反应的抗原性物质。纯化过程包括用丙酮粉提取组织、在DEAE树脂上进行分批和柱色谱、硫酸铵沉淀、在Sephadex G - 200上进行凝胶过滤以及在抗体 - Sepharose 4B上进行亲和色谱。使用鼻咽癌患者的血清对培养的人胚胎细胞进行间接免疫荧光检查，显示出斑点状核型。该抗原因子是一种可溶性酸性蛋白，等电点为5.0，分子量为250,000。来自牛胸腺的这种纯化物质以及培养的人胚胎细胞上的物质的抗原活性可被蛋白酶、核糖核酸酶和碱性磷酸酶破坏。这些决定簇对高碘酸盐氧化也敏感。证明了其在60摄氏度下的热稳定性以及在2.6至8.5之间的pH稳定性。通过免疫荧光显示了该抗原性物质与从癌症患者和自身免疫性疾病患者分离出的抗体之间的交叉反应，并进行了适当的封闭和吸收对照。
