Nomoto Y, Sakai H
J Lab Clin Med. 1979 Jul;94(1):76-87.
ANF was determined in sera from patients with IgA nephropathy to evaluate the autoimmune nature of this disorder. Sera from 33 patients with IgA nephropathy were assayed for ANF by the FANA technique at 37 degrees and 4 degrees C as well as by a hemagglutination technique. In addition, sera from five patients with lupus nephritis and six patients with idiopathic membranous nephropathy were examined. Thirty healthy adults served as controls. Cold-reacting ANF, which was detected predominantly in the class of IgM, was observed in 81.8% of patients with IgA nephropathy. The nuclear staining pattern was "speckled." Sera from healthy adults did not show any positive signs of ANF, whereas those from patients with lupus nephritis demonstrated "shaggy" pattern of ANF. It is suggested that some autoimmune mechanisms may be involved in the development of IgA nephropathy.
测定IgA肾病患者血清中的抗核因子（ANF），以评估该疾病的自身免疫性质。采用荧光抗核抗体（FANA）技术在37℃和4℃下以及血凝技术对33例IgA肾病患者的血清进行ANF检测。此外，还检测了5例狼疮性肾炎患者和6例特发性膜性肾病患者的血清。30名健康成年人作为对照。在81.8%的IgA肾病患者中观察到主要在IgM类别中检测到的冷反应性ANF。核染色模式为“斑点状”。健康成年人的血清未显示ANF的任何阳性迹象，而狼疮性肾炎患者的血清显示ANF的“蓬松”模式。提示一些自身免疫机制可能参与了IgA肾病的发生发展。
