Latchoumane Charles-Francois V, Forghani Rameen, Karumbaiah Lohitash
Animal and Dairy Science, University of Georgia, Athens, GA, USA.
Regenerative Bioscience Center, University of Georgia, Athens, GA, USA.
Bio Protoc. 2021 Nov 20;11(22):e4153. doi: 10.21769/BioProtoc.4153.
Severe traumatic brain injury (sTBI) survivors experience permanent functional disabilities due to significant volume loss and the brain's poor capacity to regenerate. Chondroitin sulfate glycosaminoglycans (CS-GAGs) are key regulators of growth factor signaling and neural stem cell homeostasis in the brain. In this protocol, we describe how to perform recordings to quantify the neuroprotective and regenerative effect of implanted engineered CS-GAG hydrogel (eCS) on brain tissue. This experiment was performed in rats under three conditions: healthy without injury (Sham), controlled cortical impact (CCI) injury on the rostral forelimb area (RFA), and CCI-RFA with eCS implants. This protocol describes the procedure used to perform the craniotomy, the positioning of the cortical recording electrode, the positioning of the stimulation electrode (contralateral paw), and the recording procedure. In addition, a description of the exact electrical setup is provided. This protocol details the recordings in the brain of injured animals while preserving most of the uninjured tissue intact, with additional considerations for intralesional and laminar recordings of multi-unit response. Graphic abstract: Sensorimotor response to paw stimulation using cortical laminar recordings.
重度创伤性脑损伤（sTBI）幸存者由于大量脑容量损失以及大脑再生能力差而经历永久性功能残疾。硫酸软骨素糖胺聚糖（CS-GAGs）是大脑中生长因子信号传导和神经干细胞稳态的关键调节因子。在本方案中，我们描述了如何进行记录以量化植入的工程化CS-GAG水凝胶（eCS）对脑组织的神经保护和再生作用。本实验在大鼠身上进行了三种条件下的操作：未受伤的健康状态（假手术组）、在 Rostral 前肢区域（RFA）进行控制性皮质撞击（CCI）损伤、以及在 CCI-RFA 损伤基础上植入 eCS。本方案描述了开颅手术的操作步骤、皮质记录电极的定位、刺激电极（对侧爪）的定位以及记录过程。此外，还提供了确切的电气设置说明。本方案详细介绍了在保留大部分未受伤组织完整的情况下对受伤动物大脑进行记录的方法，同时还考虑了对多单位反应进行病灶内和层流记录的额外注意事项。图形摘要：使用皮质层流记录对爪刺激的感觉运动反应。
