Saha Dyuti, Thannimangalath Sujaya, Budamakuntla Leelavathy, Loganathan Eswari, Jamora Colin
IFOM-inStem Joint Research Laboratory, Centre for Inflammation and Tissue Homeostasis, Institute for Stem Cell Science and Regenerative Medicine, Bangalore, India.
Department of Biology, Manipal Academy of Higher Education, Manipal, India.
JID Innov. 2021 Jul 9;1(3):100041. doi: 10.1016/j.xjidi.2021.100041. eCollection 2021 Sep.
An exploding public health crisis is the exponential growth in the incidence of chronic nonhealing ulcers associated with diseases such as diabetes. Various modalities have been developed to stimulate wound closure that is otherwise recalcitrant to standard clinical treatments. However, these approaches primarily focus on the process of re-epithelialization and are often deficient in regenerating the full spectrum of structures necessary for normal skin function. Autologous hair follicle grafting is a recent therapy to stimulate the closure of such nonhealing wounds, and we observed effects beyond the epidermis to other important components of the dermis. We found that hair follicle grafting facilitated the reappearance of various undifferentiated and differentiated layers of the epidermis with the restoration of epidermal junctions. In addition, other important structures that are critical for cutaneous health and function such as the blood and lymph vasculature, nerve fibers, and sweat gland structures were restored in postgrafted wounds. Interestingly, both immune cells and inflammatory signals were substantially decreased, indicating a reduction in the chronic inflammation that is a hallmark of nonhealing wounds. Our observation that punch wounds created on the postgrafted area likewise healed suggests that this is a self-sustaining long-term therapy for patients with chronic wounds.
一场日益严重的公共卫生危机是与糖尿病等疾病相关的慢性难愈合溃疡发病率呈指数级增长。人们已经开发出各种方法来刺激伤口愈合，而这些伤口对标准临床治疗往往反应不佳。然而，这些方法主要集中在重新上皮化过程，并且在再生正常皮肤功能所需的全部结构方面常常存在不足。自体毛囊移植是一种最近用于刺激此类难愈合伤口闭合的治疗方法，我们观察到其效果不仅限于表皮，还涉及真皮的其他重要成分。我们发现毛囊移植促进了表皮各未分化和分化层的重新出现，并恢复了表皮连接。此外，对皮肤健康和功能至关重要的其他重要结构，如血液和淋巴脉管系统、神经纤维和汗腺结构，在移植后的伤口中也得到了恢复。有趣的是，免疫细胞和炎症信号都大幅减少，这表明作为难愈合伤口标志的慢性炎症有所减轻。我们观察到在移植后区域产生的打孔伤口同样愈合，这表明这是一种适用于慢性伤口患者的自我维持的长期治疗方法。
