Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Odontologia Restauradora, Ribeirão Preto, Brasil.
Universidade de São Paulo, Faculdade de Filosofia, Ciências e Letras, Departamento de Química, Ribeirão Preto, Brasil.
J Appl Oral Sci. 2021 Dec 13;29:e20210356. doi: 10.1590/1678-7757-2021-0356. eCollection 2021.
Degradation of the dentin-resin interface can occur due to hydrolysis of exposed collagen, resulting in reduced bond strength. This study assessed the effect of dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system on improvement of bond strength and preservation of the interface durability.
Enamel was removed from 80 molars and the teeth were divided into two groups: without chitosan (control) or with 2.5% chitosan gel (1 min). They were further subdivided into two subgroups according to the adhesive system: etch-and-rinse or self-etch. Dentin was restored using a composite resin. Half of the specimens from each restored group were subjected to interface aging and the remaining specimens were used for immediate analysis. The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5). Data were analyzed using three-way ANOVA.
Chitosan improved the µTBS of the adhesive interface when compared with the control group (p=0.004). No significant differences were observed in dentin adhesion between the adhesive systems (p=0.652). Immediate µTBS was not significantly different from that after 6 months (p=0.274). EDS and SEM did not show significant differences in the chemical and structural composition of the specimens. FTIR showed a decrease in the intensity of phosphate and carbonate bands after using chitosan.
Dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system improved the immediate and preserved the 6-month bond strength of the adhesive interface.
暴露的胶原蛋白发生水解会导致牙本质-树脂界面降解，从而导致粘结强度降低。本研究评估了用壳聚糖处理牙本质，结合酸蚀-冲洗或自酸蚀粘结系统对提高粘结强度和保持界面耐久性的影响。
从 80 颗磨牙上去除釉质，将牙齿分为两组：无壳聚糖（对照组）或 2.5%壳聚糖凝胶（1 分钟）。根据粘结系统进一步分为两组：酸蚀-冲洗或自酸蚀。用复合树脂修复牙本质。每个修复组的一半样本进行界面老化，其余样本用于即时分析。将样本切割并进行微拉伸粘结强度（µTBS）测试（n=10）、傅里叶变换红外（FTIR）光谱化学组成测试（n=4）和能量色散光谱（EDS）（n=5）、以及扫描电子显微镜（SEM）（n=5）对粘结界面的形态分析。使用三因素方差分析对数据进行分析。
与对照组相比，壳聚糖提高了粘结界面的µTBS（p=0.004）。两种粘结系统之间牙本质粘结无显著差异（p=0.652）。即时µTBS与 6 个月后无显著差异（p=0.274）。EDS 和 SEM 未显示标本化学和结构组成有显著差异。FTIR 显示使用壳聚糖后磷酸盐和碳酸盐带的强度降低。
用壳聚糖处理牙本质，结合酸蚀-冲洗或自酸蚀粘结系统，可提高粘结界面的即时和 6 个月粘结强度。
