壳聚糖和 CMCS 对 Er:YAG 激光照射后牙本质的影响：剪切粘结强度和表面形貌分析。

Effect of chitosan and CMCS on dentin after Er:YAG laser irradiation: shear bond strength and surface morphology analysis.

机构信息

Department of Endodontics, School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction & Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou Medical University, Guangzhou, Guangdong, 510182, China.

出版信息

BMC Oral Health. 2024 Mar 29;24(1):402. doi: 10.1186/s12903-024-04097-w.

DOI:10.1186/s12903-024-04097-w

PMID:38553692

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10979601/

Abstract

OBJECTIVES

The aim of the present study was to evaluate the effect of chitosan and carboxymethyl chitosan (CMCS) on dentin surface morphology and bonding strength after irradiation of Er:YAG laser.

METHODS

Eighty-four laser-irradiated dentin samples were randomly distributed into three groups (n = 28/group) according to different surface conditioning process: deionized water for 60s; 1wt% chitosan for 60s; or 1wt% CMCS for 60s. Two specimens from each group were subjected to TEM analysis to confirm the presence of extrafibrillar demineralization on dentin fibrils. Two specimens from each group were subjected to morphological analysis by SEM. Seventy-two specimens (n = 24/group) were prepared, with a composite resin cone adhered to the dentin surface, and were then randomly assigned to one of two aging processes: storage in deionized water for 24 h or a thermocycling stimulation. The shear bond strength of laser-irradiated dentin to the resin composite was determined by a universal testing machine. Data acquired in the shear bond strength test was analyzed by one-way ANOVA with the Tukey honestly significant difference post hoc test and Independent Samples t-test (α = 0.05).

RESULTS

CMCS group presented demineralized zone and a relatively smooth dentin surface morphology. CMCS group had significantly higher SBS value (6.08 ± 2.12) without aging (p < 0.05). After thermal cycling, both chitosan (5.26 ± 2.30) and CMCS group (5.82 ± 1.90) presented higher bonding strength compared to control group (3.19 ± 1.32) (p < 0.05). Chitosan and CMCS group preserved the bonding strength after aging process (p > 0.05).

CONCLUSIONS

CMCS has the potential to be applied in conjunction with Er:YAG laser in cavity preparation and resin restoration.

摘要

目的

本研究旨在评估壳聚糖和羧甲基壳聚糖（CMCS）对 Er：YAG 激光照射后牙本质表面形态和粘结强度的影响。

方法

将 84 个激光照射后的牙本质样本随机分为三组（n=28/组），根据不同的表面处理方式：去离子水处理 60s；1wt%壳聚糖处理 60s；或 1wt%CMCS 处理 60s。每组的两个样本进行 TEM 分析以确认牙本质纤维上有无额外的纤维间脱矿。每组的两个样本进行 SEM 形态分析。制备 72 个样本（n=24/组），将复合树脂锥体粘接到牙本质表面，然后将其随机分为两种老化处理之一：在去离子水中储存 24 小时或热循环刺激。通过万能试验机测定激光照射后的牙本质与树脂复合材料的剪切粘结强度。用单因素方差分析和 Tukey Honestly Significant Difference 事后检验和独立样本 t 检验（α=0.05）分析剪切粘结强度测试中的数据。

结果

CMCS 组牙本质表面呈现脱矿区和相对光滑的形态。CMCS 组未经老化处理时的 SBS 值显著更高（6.08±2.12）（p<0.05）。经过热循环后，壳聚糖（5.26±2.30）和 CMCS 组（5.82±1.90）的粘结强度均高于对照组（3.19±1.32）（p<0.05）。壳聚糖和 CMCS 组在老化后保持了粘结强度（p>0.05）。