Ezban M, Wallevik K, Hansen J F
Dev Biol Stand. 1980;48:83-92.
In plasma fractionation industry different methods are used for producing albumin for therapeutic use. We wanted to investigate whether the different ways of fractionating plasma have an effect on the nativity of the albumin molecules. Isoelectric focusing in 6M urea has shown the presence of variable amounts of oxidized albumin in different preparations. The presence of oxidized forms indicates that an initial denaturation has taken place. To evaluate if the alteration in the albumin molecules has any effects in vivo, metabolic studies in rabbits and humans with radioiodinated albumin were undertaken. Our preliminary results show a correlation between the in vivo metabolic studies and the amount of oxidized albumin.
在血浆分级分离行业中，使用不同的方法来生产用于治疗的白蛋白。我们想要研究血浆分级分离的不同方式是否会对白蛋白分子的天然状态产生影响。在6M尿素中进行等电聚焦已表明不同制剂中存在不同量的氧化型白蛋白。氧化型的存在表明已经发生了初始变性。为了评估白蛋白分子的改变在体内是否有任何影响，对兔子和人类进行了用放射性碘化白蛋白的代谢研究。我们的初步结果显示体内代谢研究与氧化型白蛋白的量之间存在相关性。
