State Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, Shandong 250353, P.R. China.
Shandong Provincial Key Laboratory of Food and Fermentation Engineering, Shandong Food Ferment Industry Research and Design Institute, Qilu University of Technology, Shandong Academy of Sciences, Jinan, Shandong 250013, P.R. China.
Mol Med Rep. 2022 Feb;25(2). doi: 10.3892/mmr.2021.12568. Epub 2021 Dec 16.
Ulcerative colitis (UC) is a global disease, characterized by periods of relapse that seriously affects the quality of life of patients. Oligosaccharides are considered to be a prospective strategy to alleviate the symptoms of UC. The present study aimed to evaluate the effect of weilan gum oligosaccharide (WLGO) on a mouse UC model induced by dextran sulfate sodium (DSS). WLGO structural physical properties were characterized by electrospray mass spectrometry and fourier tansform infrared spectroscopy. MTT assays were performed to evaluate the non‑toxic concentration of WLGO. RT‑qPCR and ELISAs were conducted to determine the levels of inflammatory factors. The clinical symptoms and mucosal integrity of the DSS‑induced UC model were assessed by DAI and histological assessment. LPS‑induced Caco‑2 cells and DSS‑induced UC mice were used to explore the effects of WLGO on UC. Treatment of the mice with 4.48 g/kg/day WLGO via gavage for 7 days significantly relieved the symptoms of DSS‑induced UC model mice, whereas significant effects were not observed for all symptoms of DSS‑induced UC in the WLGO‑low group. The disease activity index score was decreased and the loss of body weight was reduced in DSS‑induced UC model mice treated with WLGO. Moreover, colonic damage and abnormally short colon length shortenings were relieved following WLGO treatment. WLGO treatment also reduced the concentration and mRNA expression levels of proinflammatory cytokines, including interleukin‑1β, interleukin‑6 and tumor necrosis factor α, in DSS‑induced UC model mice and lipopolysaccharide‑treated Caco‑2 cells. These results indicated that WLGO may be an effective strategy for UC treatment.
溃疡性结肠炎（UC）是一种全球性疾病，其特点是反复发作，严重影响患者的生活质量。低聚糖被认为是一种缓解 UC 症状的有前途的策略。本研究旨在评估槐糖脂低聚糖（WLGO）对葡聚糖硫酸钠（DSS）诱导的小鼠 UC 模型的影响。采用电喷雾质谱和傅里叶变换红外光谱对 WLGO 的结构物理性质进行了表征。通过 MTT 测定法评估 WLGO 的无毒浓度。通过 RT-qPCR 和 ELISA 测定炎症因子的水平。通过 DAI 和组织学评估评估 DSS 诱导的 UC 模型的临床症状和黏膜完整性。使用 LPS 诱导的 Caco-2 细胞和 DSS 诱导的 UC 小鼠来探索 WLGO 对 UC 的影响。通过灌胃 7 天每天给予 4.48 g/kg 的 WLGO 可显著缓解 DSS 诱导的 UC 模型小鼠的症状，而 WLGO-低剂量组对 DSS 诱导的 UC 的所有症状均无显著作用。DSS 诱导的 UC 模型小鼠的疾病活动指数评分降低，体重减轻减少。此外，WLGO 治疗还缓解了结肠损伤和异常缩短的结肠长度。WLGO 治疗还降低了 DSS 诱导的 UC 模型小鼠和脂多糖处理的 Caco-2 细胞中促炎细胞因子（包括白细胞介素-1β、白细胞介素-6 和肿瘤坏死因子-α）的浓度和 mRNA 表达水平。这些结果表明，WLGO 可能是治疗 UC 的有效策略。
