Qiu Kai, Li Cheng-Liang, Wang Jing, Qi Guang-Hai, Gao Jun, Zhang Hai-Jun, Wu Shu-Geng
Risk Assessment Laboratory of Feed Derived Factors to Animal Product Quality Safety of Ministry of Agriculture and Rural Affairs, National Engineering Research Center of Biological Feed, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China.
Animal Nutrition, Nutrition and Care, Evonik (China) Co., Ltd., Beijing, China.
Front Nutr. 2021 Nov 29;8:786878. doi: 10.3389/fnut.2021.786878. eCollection 2021.
() as in-feed probiotics is a potential alternative for antibiotic growth promoters (AGP) in the poultry industry. The current study investigated the effects of on the performance, immunity, gut microbiota, and intestinal barrier function of broiler chickens. A 42-day feeding trial was conducted with a total of 600 1-day-old Arbor Acres broilers with similar initial body weight, which was randomly divided into one of five dietary treatments: the basal diet (Ctrl), Ctrl + virginiamycin (AGP), Ctrl + A (BSA), Ctrl + B (BSB), and Ctrl + A + B (1:1, BSAB). The results showed significantly increased average daily gain in a step-wise manner from the control, , and to the AGP groups. The mortality rate of the group was significantly lower than the AGP group. The concentrations of serum immunoglobulin (Ig) G (IgG), IgA, and IgM in the and AGP groups were higher than the control group, and the groups had the highest content of serum lysozyme and relative weight of thymus. Dietary increased the relative length of ileum and the relative weight of jejunum compared with the AGP group. The villus height (V), crypt depth (C), V/C, and intestinal wall thickness of the jejunum in the and AGP groups were increased relative to the control group. Dietary increased the messenger RNA (mRNA) expression of ZO-1, Occludin, and Claudin-1, the same as AGP. The contents of lactic acid, succinic acid, and butyric acid in the ileum and cecum were increased by dietary . Dietary significantly increased the and in the ileum and cecum and decreased the and in the cecum. The improved performance and decreased mortality rate observed in the feeding trial could be accrued to the positive effects of on the immune response capacity, gut health, and gut microflora balance, and the combination of two strains showed additional benefits on the intestinal morphology and tight junction protein expressions. Therefore, it can be concluded that dietary A and B could be used as alternatives to synthetic antibiotics in the promotion of gut health and productivity index in broiler production.
（）作为饲料中的益生菌是家禽业抗生素生长促进剂（AGP）的一种潜在替代品。本研究调查了（此处原文缺失具体物质）对肉鸡生产性能、免疫力、肠道微生物群和肠道屏障功能的影响。对总共600只初始体重相似的1日龄艾维茵肉鸡进行了为期42天的饲养试验，将其随机分为五种日粮处理之一：基础日粮（对照）、对照+维吉尼亚霉素（AGP）、对照+（此处原文缺失具体物质）A（BSA）、对照+（此处原文缺失具体物质）B（BSB）以及对照+（此处原文缺失具体物质）A+B（1:1，BSAB）。结果显示，从对照组、（此处原文缺失具体物质）组和（此处原文缺失具体物质）组到AGP组，平均日增重呈逐步显著增加。（此处原文缺失具体物质）组的死亡率显著低于AGP组。（此处原文缺失具体物质）组和AGP组血清免疫球蛋白（Ig）G（IgG）、IgA和IgM的浓度高于对照组，（此处原文缺失具体物质）组血清溶菌酶含量和胸腺相对重量最高。与AGP组相比，日粮（此处原文缺失具体物质）增加了回肠的相对长度和空肠的相对重量。（此处原文缺失具体物质）组和AGP组空肠的绒毛高度（V）、隐窝深度（C）、V/C和肠壁厚度相对于对照组增加。日粮（此处原文缺失具体物质）增加了紧密连接蛋白ZO-1、闭合蛋白和Claudin-1的信使核糖核酸（mRNA）表达，与AGP相同。日粮（此处原文缺失具体物质）增加了回肠和盲肠中乳酸、琥珀酸和丁酸的含量。日粮（此处原文缺失具体物质）显著增加了回肠和盲肠中的（此处原文缺失具体物质）和（此处原文缺失具体物质），并降低了盲肠中的（此处原文缺失具体物质）和（此处原文缺失具体物质）。饲养试验中观察到的生产性能改善和死亡率降低可能归因于（此处原文缺失具体物质）对免疫反应能力、肠道健康和肠道微生物群平衡的积极影响，并且两种菌株的组合对肠道形态和紧密连接蛋白表达显示出额外的益处。因此，可以得出结论，日粮（此处原文缺失具体物质）A和B可作为合成抗生素的替代品，用于促进肉鸡生产中的肠道健康和生产性能指标。
