Grzybowska Dominika, Snarska Anna
Department and Clinic of Internal Diseases Faculty of Veterinary Medicine University of Warmia and Mazury, 10-719 Olsztyn, Poland.
J Vet Res. 2021 Jul 22;65(3):323-327. doi: 10.2478/jvetres-2021-0040. eCollection 2021 Sep.
Due to the widely documented and diverse toxic effects of acrylamide, the authors decided to evaluate the impact of high and low doses of this compound on the process of granulopoiesis in porcine bone marrow.
The experiment was conducted on 15 Danish Landrace pigs at the age of 8 weeks. The animals were randomly assigned into three equal groups (n = 5). Control animals received empty gelatine capsules as placebo. Animals in the first experimental group (the LD group) received a low dose of acrylamide of 0.5 μg/kg b.w./day, and animals in the second experimental group (the HD group) received a tenfold higher dose of acrylamide of 5 μg/kg b.w./day. Placebo and acrylamide capsules were administered with feed every morning for 28 days. Bone marrow was collected into tubes without an anticoagulant twice - before the first capsule administration (day 0) and on the 28 day of the study. After drying and staining, bone marrow smears were subjected to detailed cytological evaluation under a light microscope.
Changes in cell morphology, . degenerative changes in the cellular nuclei, were observed in both experimental groups. Both low and high doses of acrylamide decreased the number of segmented eosinophils, neutrophilic and segmented metamyelocytes, neutrophils, as well as basophils and basophilic metamyelocytes.
Acrylamide at doses of 0.5 μg/kg b.w./day and 5 μg/kg b.w./day clearly influences porcine granulopoiesis.
由于丙烯酰胺具有广泛记载的多样毒性作用，作者决定评估该化合物的高剂量和低剂量对猪骨髓粒细胞生成过程的影响。
实验在15头8周龄的丹麦长白猪身上进行。动物被随机分为三组，每组5头。对照组动物接受空明胶胶囊作为安慰剂。第一实验组（低剂量组）的动物接受0.5μg/kg体重/天的低剂量丙烯酰胺，第二实验组（高剂量组）的动物接受10倍剂量即5μg/kg体重/天的丙烯酰胺。每天早晨将安慰剂和丙烯酰胺胶囊与饲料一起投喂，持续28天。在首次投喂胶囊前（第0天）和研究的第28天，两次将骨髓收集到不含抗凝剂的试管中。干燥和染色后，在光学显微镜下对骨髓涂片进行详细的细胞学评估。
在两个实验组中均观察到细胞形态的变化，即细胞核的退行性变化。低剂量和高剂量的丙烯酰胺均减少了分叶嗜酸性粒细胞、中性粒细胞和分叶晚幼粒细胞、中性粒细胞以及嗜碱性粒细胞和嗜碱性晚幼粒细胞的数量。
0.5μg/kg体重/天和5μg/kg体重/天剂量的丙烯酰胺明显影响猪的粒细胞生成。
