Yener Y, Dikmenli M
Department of Biology Education, Faculty of Ahmet Keleşoğlu Education, Selcuk University, 42090 Konya, Turkey.
Food Chem Toxicol. 2009 Aug;47(8):2120-3. doi: 10.1016/j.fct.2009.05.037. Epub 2009 Jun 14.
This study was performed to investigate whether acrylamide (AA), occurred during cooking carbohydrate-rich foods at high temperature, increased the frequency of micronucleated polychromatic erythrocytes (MNPCEs) in rat bone marrow. For this purpose AA, dissolved in distilled water, was administered to 8-week old male Sprague Dawley rats at single oral doses of 0, 125, 150 or 175 mg/kg b.w. After 48 h from AA treatment, the bone marrow samples were analysed for the frequency of MNPCEs. The cytotoxic effect of AA on bone marrow was also tested by assessing polychromatic erythrocyte/normochromatic erythrocyte (PCE/NCE) ratio. It was found that all three doses applied significantly increased the frequency of MNPCEs and this increase was 3.75-fold in rats given the highest administered dose of AA. In addition AA decreased the PCE/NCE ratio, which is indicative of bone marrow cytotoxicity when compared to the control group. This study displayed that AA increased the formation of micronuclei in polychromatic erythrocytes (PCEs) of rat bone marrow and this increase might have resulted from administrating the high dose level of AA to rats by gavage instead of by i.p. injection.
本研究旨在调查高温烹饪富含碳水化合物的食物过程中产生的丙烯酰胺（AA）是否会增加大鼠骨髓中微核多染红细胞（MNPCEs）的频率。为此，将溶于蒸馏水的AA以0、125、150或175mg/kg体重的单次口服剂量给予8周龄雄性斯普拉格-道利大鼠。在给予AA处理48小时后，分析骨髓样本中MNPCEs的频率。还通过评估多染红细胞/正染红细胞（PCE/NCE）比率来测试AA对骨髓的细胞毒性作用。结果发现，所应用的所有三个剂量均显著增加了MNPCEs的频率，在给予最高剂量AA的大鼠中，这种增加为3.75倍。此外，AA降低了PCE/NCE比率，与对照组相比，这表明存在骨髓细胞毒性。本研究表明，AA增加了大鼠骨髓多染红细胞（PCEs）中微核的形成，这种增加可能是由于通过灌胃而非腹腔注射给大鼠施用高剂量水平的AA所致。
