白细胞介素4是小鼠结缔组织型肥大细胞体外克隆生长的必需因子。
Interleukin 4 as an essential factor for in vitro clonal growth of murine connective tissue-type mast cells.
作者信息
Hamaguchi Y, Kanakura Y, Fujita J, Takeda S, Nakano T, Tarui S, Honjo T, Kitamura Y
出版信息
J Exp Med. 1987 Jan 1;165(1):268-73. doi: 10.1084/jem.165.1.268.
Abstract
We investigated the biological activity of IL-4 to murine connective tissue-type mast cells (CTMC). When purified peritoneal mast cells, typical CTMC, were incubated with pokeweed mitogen-stimulated spleen cell-conditioned medium (PWM-SCM) in methylcellulose, about one-fifth of mast cells showed clonal growth. Recombinant IL-4 alone did not stimulate the clonal growth, and purified IL-3 alone induced development of a small number of tiny clusters. In contrast, addition of IL-4 to IL-3 increased the number of clusters by a factor of 10. The number and size of clusters induced by the combination of IL-3 and IL-4 were comparable to those of mast cell clusters induced by PWM-SCM. The present results indicate that IL-4 is an essential factor for in vitro clonal growth of CTMC.
摘要
我们研究了白细胞介素-4（IL-4）对小鼠结缔组织型肥大细胞（CTMC）的生物学活性。当将典型的CTMC——纯化的腹膜肥大细胞与商陆丝裂原刺激的脾细胞条件培养基（PWM-SCM）在甲基纤维素中孵育时，约五分之一的肥大细胞呈现克隆生长。单独的重组IL-4不刺激克隆生长，单独的纯化IL-3仅诱导少量微小细胞簇的形成。相反，在IL-3中添加IL-4可使细胞簇数量增加10倍。IL-3和IL-4组合诱导的细胞簇数量和大小与PWM-SCM诱导的肥大细胞簇相当。目前的结果表明，IL-4是CTMC体外克隆生长的必需因子。
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