Department of Biomolecular Systems, Max-Planck-Institute of Colloids and Interfaces, 14476, Potsdam, Germany.
Department of Biomolecular Systems, Max-Planck-Institute of Colloids and Interfaces, 14476, Potsdam, Germany; Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195, Berlin, Germany.
Carbohydr Res. 2022 Jan;511:108489. doi: 10.1016/j.carres.2021.108489. Epub 2021 Dec 11.
Currently, the reaction toolbox for the functionalization of glycans assembled on solid-phase is quite limited. Automated (1 h) and manual (overnight) phosphorylation protocols that enable the solid-phase synthesis of oligosaccharides containing up to two mannose-6-phosphates are presented. Automated glycan assembly expedited access to substrates and facilitated the screening of experimental conditions.
目前，用于固相组装的聚糖功能化的反应工具箱相当有限。本文提出了两种自动化（1 小时）和手动（过夜）的磷酸化方案，可在固相合成含有多达两个甘露糖-6-磷酸的寡糖。自动化聚糖组装加快了底物的获取，并促进了实验条件的筛选。
