Department of Chemistry and Biochemistry, University of Missouri - St. Louis, One University Boulevard, St. Louis, Missouri, 63121, USA.
Department of Chemistry, Saint Louis University, 3501 Laclede Ave, St. Louis, Missouri, 63103, USA.
Chemistry. 2022 Jul 11;28(39):e202201180. doi: 10.1002/chem.202201180. Epub 2022 May 25.
As the 21 century unfolds with rapid changes, new challenges in research and development emerge. These new challenges prompted us to repurpose our HPLC-A platform that was previously used in solid phase glycan synthesis to a solution phase batch synthesis described herein. The modular character of HPLC allows for implementing new attachments. To enable sequential synthesis of multiple oligosaccharides with the single press of a button, we supplemented our system with a four-way split valve and an automated fraction collector. This enabled the operator to load all reagents and all reactants in the autosampler, press the button to start the repetitive automation sequence, leave the lab, and upon return find products of multiple reactions ready for purification, analysis, and subsequent application.
随着 21 世纪的快速发展，研究和开发出现了新的挑战。这些新的挑战促使我们重新利用之前用于固相糖合成的 HPLC-A 平台，将其改为本文所述的溶液相批量合成。HPLC 的模块化特点允许实现新的附件。为了能够通过单次按下按钮顺序合成多种寡糖，我们在系统中添加了一个四路分流阀和一个自动化馏分收集器。这使得操作人员能够在自动进样器中加载所有试剂和所有反应物，按下按钮启动重复自动化序列，然后离开实验室，返回后即可获得多种反应的产物，准备进行纯化、分析和后续应用。
