连接体、负载和反应规模会影响自动化聚糖组装。
Linker, loading, and reaction scale influence automated glycan assembly.
作者信息
Dal Colle Marlene C S, Ricardo Manuel G, Hribernik Nives, Danglad-Flores José, Seeberger Peter H, Delbianco Martina
机构信息
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany.
Department of Chemistry and Biochemistry, Freie Universität Berlin, Arnimallee 22, 14195 Berlin, Germany.
出版信息
Beilstein J Org Chem. 2023 Jul 6;19:1015-1020. doi: 10.3762/bjoc.19.77. eCollection 2023.
Automated glycan assembly (AGA) affords collections of well-defined glycans in a short amount of time. We systematically analyzed how parameters connected to the solid support affect the AGA outcome for three different glycan sequences. We showed that, while loading and reaction scale did not significantly influence the AGA outcome, the chemical nature of the linker dramatically altered the isolated yields. We identified that the major determinants of AGA yields are cleavage from the solid support and post-AGA purification steps.
自动化聚糖组装（AGA）能够在短时间内提供一系列结构明确的聚糖。我们系统地分析了与固相载体相关的参数如何影响三种不同聚糖序列的 AGA 结果。我们发现，虽然负载量和反应规模对 AGA 结果没有显著影响，但连接子的化学性质会显著改变分离产率。我们确定，AGA 产率的主要决定因素是从固相载体上的切割以及 AGA 后的纯化步骤。
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