Department of Forensic Toxicology, Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Academy of Forensic Science, Shanghai 200063, China.
Department of Forensic Toxicology, Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Academy of Forensic Science, Shanghai 200063, China.
Forensic Sci Int. 2022 Feb;331:111139. doi: 10.1016/j.forsciint.2021.111139. Epub 2021 Dec 4.
In recent years, an increasing number of new synthetic cannabinoids have appeared on the drug trade market. Many of the new synthetic cannabinoids have not previously been reported. At present, there are relatively few methods available for detecting synthetic cannabinoids and their metabolites in hair matrices. Therefore, we established a simple and fast method to simultaneously identify 29 synthetic cannabinoids and their metabolites in human hair by UPLC-MS/MS. Twenty milligrams of hair was used and processed by cryo-grinding and extraction with methanol. A Waters Acquity UPLC HSS T column (100 mm × 2.1 mm, 1.8 µm) was used for chromatographic separation. Mobile phase A was comprised of 20 mmol/L ammonium acetate, 0.1% formic acid, and 5% acetonitrile and water, and mobile phase B was acetonitrile. The method was fully validated and proved to have good selectivity, accuracy, precision, and satisfactory linearity within the calibrated range. The limit of detection (LOD) ranged from 0.5 to 5 pg/mg, and the lower limit of quantitation (LLOQ) ranged from 1 to 10 pg/mg. The extraction recovery was 36.1-93.3%, and the matrix effect was 19.1-110.0%. The validated method was successfully used to qualitatively and quantitatively analyze 29 synthetic cannabinoids and their metabolites in 59 actual hair samples. MDMB-4en-PINACA had the highest positive detection rate followed by ADB-BUTINACA, and there are multiple synthetic cannabinoid mixed ingestions. This methodology has great potential for the detection of 29 synthetic cannabinoids and their metabolites in forensic cases.
近年来，越来越多的新型合成大麻素出现在毒品交易市场上。其中许多新型合成大麻素以前并未有过报道。目前，用于检测毛发基质中合成大麻素及其代谢物的方法相对较少。因此，我们建立了一种简单、快速的超高效液相色谱-串联质谱法（UPLC-MS/MS）同时检测人发中 29 种合成大麻素及其代谢物的方法。取 20mg 毛发，经冷冻研磨后，用甲醇提取处理。采用 Waters Acquity UPLC HSS T 柱（100mm×2.1mm，1.8μm）进行色谱分离。流动相 A 由 20mmol/L 乙酸铵、0.1%甲酸和 5%乙腈-水组成，流动相 B 为乙腈。该方法经过全面验证，具有良好的选择性、准确性、精密度和令人满意的线性范围。检测限（LOD）范围为 0.5-5pg/mg，定量下限（LLOQ）范围为 1-10pg/mg。提取回收率为 36.1-93.3%，基质效应为 19.1-110.0%。该验证方法成功地用于定性和定量分析 59 份实际毛发样品中的 29 种合成大麻素及其代谢物。MDMB-4en-PINACA 的阳性检出率最高，其次是 ADB-BUTINACA，存在多种合成大麻素混合摄入的情况。该方法在法医案例中检测 29 种合成大麻素及其代谢物具有很大的潜力。
