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从小鼠小肠上皮分离的淋巴细胞的功能特性。

Functional properties of lymphocytes isolated from murine small intestinal epithelium.

作者信息

Dillon S B, MacDonald T T

出版信息

Immunology. 1984 Jul;52(3):501-9.

PMID:6611297
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1454501/
Abstract

Intraepithelial lymphocytes (IEL) were isolated from murine small intestine using a modification of previously published procedures. Analysis of IEL by immunofluorescence using monoclonal antibodies showed they were predominantly Lyt-2+ cells, with relatively few B cells or macrophages present. IEL cultured at sufficiently high cell densities proliferated in response to concanavalin A (Con A), phytohaemagglutin (PHA) and lipopolysaccharide (LPS). IEL were also capable of recognizing alloantigens in an in vivo graft-versus-host assay and in an in vitro mixed lymphocyte reaction. These studies therefore confirm that murine IEL contain cells with immunologic properties characteristic of typical T lymphocytes.

摘要

采用对先前发表方法的改良，从小鼠小肠中分离出上皮内淋巴细胞（IEL）。使用单克隆抗体通过免疫荧光对IEL进行分析，结果显示它们主要是Lyt-2+细胞，B细胞或巨噬细胞相对较少。以足够高的细胞密度培养的IEL会对刀豆球蛋白A（Con A）、植物血凝素（PHA）和脂多糖（LPS）产生增殖反应。IEL在体内移植物抗宿主试验和体外混合淋巴细胞反应中也能够识别同种异体抗原。因此，这些研究证实小鼠IEL含有具有典型T淋巴细胞免疫特性的细胞。

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1
Functional properties of lymphocytes isolated from murine small intestinal epithelium.从小鼠小肠上皮分离的淋巴细胞的功能特性。
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2
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Functional characterization of Con A-responsive Lyt2-positive mouse small intestinal intraepithelial lymphocytes.伴刀豆球蛋白A反应性Lyt2阳性小鼠小肠上皮内淋巴细胞的功能特性
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本文引用的文献

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Characteristics of natural killer cells in the murine intestinal epithelium and lamina propria.小鼠肠道上皮和固有层中自然杀伤细胞的特征
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Isolation and functional characteristics of adherent phagocytic cells from mouse Peyer's patches.从小鼠派伊尔结中分离出的贴壁吞噬细胞及其功能特性
Immunology. 1982 Apr;45(4):769-74.
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Intraepithelial, lamina propria and Peyer's patch lymphocytes of the rat small intestine: isolation and characterization in terms of immunoglobulin markers and receptors for monoclonal antibodies.
L-精氨酸可刺激经传染性法氏囊病疫苗活中等加毒株口服免疫的鸡的肠道上皮内淋巴细胞功能和免疫反应。
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Phenotypic and functional heterogeneity of murine intestinal intraepithelial lymphocytes defined by cell density: implications for route of differentiation and responsiveness to proliferation induction.根据细胞密度定义的小鼠肠道上皮内淋巴细胞的表型和功能异质性：对分化途径和增殖诱导反应性的影响
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Regional variation in the proliferative rate and lifespan of alpha beta TCR+ and gamma delta TCR+ intraepithelial lymphocytes in the murine small intestine.小鼠小肠中αβTCR⁺和γδTCR⁺上皮内淋巴细胞增殖率和寿命的区域差异。
Immunology. 1995 Oct;86(2):212-8.
7
Frequency of occurrence and distribution of the intra-epithelial lymphoid cells in the follicle-associated epithelium in phenotypically normal and athymic nude mice.表型正常和无胸腺裸鼠中滤泡相关上皮内上皮淋巴细胞的发生频率和分布
J Anat. 1987 Jun;152:121-31.
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T cell subclasses in fetal human ileum.人类胎儿回肠中的T细胞亚类。
Clin Exp Immunol. 1986 Sep;65(3):553-8.
9
Functional characteristics of intraepithelial lymphocytes from mouse small intestine. III. Inability of intraepithelial lymphocytes to induce a systemic graft-versus-host reaction is because of failure to migrate in vivo.小鼠小肠上皮内淋巴细胞的功能特性。III. 上皮内淋巴细胞无法诱导全身性移植物抗宿主反应是由于其在体内无法迁移。
Gut. 1987 Oct;28(10):1267-74. doi: 10.1136/gut.28.10.1267.
10
Responses of intraepithelial lymphocytes to T-cell mitogens: a comparison between murine and porcine responses.上皮内淋巴细胞对T细胞有丝分裂原的反应：小鼠和猪反应的比较。
Immunology. 1986 Aug;58(4):621-5.
大鼠小肠上皮内、固有层和派尔集合淋巴结淋巴细胞：根据免疫球蛋白标记物和单克隆抗体受体进行分离与特性鉴定
Immunology. 1982 Apr;45(4):775-83.
4
Preparation and purification of lymphocytes from the epithelium and lamina propria of murine small intestine.从小鼠小肠上皮和固有层制备及纯化淋巴细胞。
Gut. 1981 Jun;22(6):481-8. doi: 10.1136/gut.22.6.481.
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The influence of gut function on lymphoid cell populations in the intestinal mucosa of lambs.肠道功能对羔羊肠黏膜淋巴样细胞群体的影响。
Immunology. 1983 Jul;49(3):501-9.
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Expression of immunoglobulin isotypes by lymphoid cells of mouse intestinal lamina propria.小鼠肠道固有层淋巴样细胞免疫球蛋白同种型的表达
Cell Immunol. 1982 Nov 1;73(2):324-36. doi: 10.1016/0008-8749(82)90459-2.
7
Immunohistologic and immunoelectron microscopic characterization of the mucosal lymphocytes of human small intestine by the use of monoclonal antibodies.利用单克隆抗体对人小肠黏膜淋巴细胞进行免疫组织学和免疫电子显微镜特征分析。
J Immunol. 1983 Jun;130(6):2615-22.
8
T lymphocyte subsets in human intestinal mucosa: the distribution and relationship to MHC-derived antigens.人类肠道黏膜中的T淋巴细胞亚群：分布及其与主要组织相容性复合体（MHC）衍生抗原的关系
Clin Exp Immunol. 1981 Jun;44(3):453-8.
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Granular intraepithelial lymphocytes of the rat small intestine. I. Isolation, presence in T lymphocyte-deficient rats and bone marrow origin.大鼠小肠的颗粒状上皮内淋巴细胞。I. 分离、在T淋巴细胞缺陷大鼠中的存在及骨髓起源
Int Arch Allergy Appl Immunol. 1983;71(4):317-27. doi: 10.1159/000233414.
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