Department of Biological Sciences, University of Limerick, Ireland; Health Research Institute, University of Limerick, Ireland; Bernal Institute, University of Limerick, Ireland. Electronic address: https://www.twitter.com/bioflips.
Section of Biotechnology and Genetics, Department of Biology, National and Kapodistrian University of Athens, Greece.
Prostaglandins Other Lipid Mediat. 2022 Feb;158:106606. doi: 10.1016/j.prostaglandins.2021.106606. Epub 2021 Dec 16.
In the present work the entomopathogenic fungus B. bassiana lipids were studied against the potent pro-inflammatory and thrombotic mediators implicated in several disorders, platelet-activating factor (PAF) and thrombin. Bioactivities of lipid extracts from B. bassiana cells and culture supernatants and of their lipid fractions separated by a one-step HPLC-analysis ere assessed against the PAF/Thrombin-induced aggregation of washed rabbit platelets. Lipid extracts from both cell-biomass and supernatant inhibited strongly PAF/Thrombin-activities and platelet-aggregation, exhibiting higher specificity against PAF. Similarly, HPLC-derived lipid-fractions of phenolics/glycolipids, Sphingomyelins and Phosphatidylcholines (PC) showed strong anti-PAF potency. PC PAF-like molecules exhibited the strongest antagonistic anti-PAF effects, while in higher amounts they agonistically inhibited PAF-activities. Some bioactive lipids with strong anti-PAF effects are exo-cellularly secreted in the culture media during fungal growth, while others are not. The presence of such lipid bioactives in B. bassiana with strong anti-inflammatory and anti-thrombotic properties, provide new perspectives and putative future applications for this entomopathogenic fungus.
在本工作中，研究了昆虫病原真菌 B. bassiana 的脂质对几种疾病中涉及的强效促炎和促血栓形成介质（血小板激活因子 [PAF] 和凝血酶）的作用。通过一步 HPLC 分析从 B. bassiana 细胞和培养上清液及其脂质级分中分离出的脂质提取物的生物活性，评估了它们对兔血小板 PAF/凝血酶诱导聚集的抑制作用。来自细胞生物量和上清液的脂质提取物强烈抑制 PAF/凝血酶活性和血小板聚集，对 PAF 表现出更高的特异性。同样，HPLC 衍生的酚类/糖脂、鞘磷脂和磷脂酰胆碱（PC）脂质级分显示出很强的抗 PAF 作用。PC PAF 样分子表现出最强的抗 PAF 作用，而在较高浓度下它们则具有激动性地抑制 PAF 活性。一些具有强抗 PAF 作用的生物活性脂质在真菌生长过程中被细胞外分泌到培养基中，而其他脂质则没有。B. bassiana 具有如此强的抗炎和抗血栓形成特性，其中存在这些脂质生物活性物质，为这种昆虫病原真菌提供了新的前景和潜在的未来应用。
