Marandykina-Prakienė Alina, Butkauskas Dalius, Gudiškis Naglis, Juozaitytė-Ngugu Evelina, Bagdonaitė Dovilė Laisvūnė, Kirjušina Muza, Calero-Bernal Rafael, Prakas Petras
Nature Research Centre, Akademijos 2, 08412 Vilnius, Lithuania.
Institute of Life Sciences and Technology, Daugavpils University, Parādes Street 1A, 5401 Daugavpils, Latvia.
Vet Sci. 2023 Aug 11;10(8):520. doi: 10.3390/vetsci10080520.
Contradictory data is available on the intermediate host specificity of spp. in farm animals. Therefore, the current work aimed at molecularly testing samples of sheep and goats reared in Lithuania to identify species described in other intermediate hosts but suspected to be non-canonical parasites to these small ruminants. For this purpose, muscle samples from 47 domestic sheep and nine goats were examined. species were identified using direct and nested PCR targeting and sequencing of positive amplified products. Along with the detection of the canonical spp. in their respective intermediate hosts, the DNA of . and . was detected in sheep, although these species were previously thought to be specific to goats and deer, respectively. In addition, DNA from . and . was found in goats, even though these two species were believed to be sheep-specific. Notably, under light microscopy, only sarcocysts of . specific to goats were observed. Thus, future research on the life cycle and host-specificity of spp. examined is warranted.
关于家畜中 spp. 的中间宿主特异性存在相互矛盾的数据。因此，当前的工作旨在对在立陶宛饲养的绵羊和山羊样本进行分子检测，以鉴定在其他中间宿主中描述但被怀疑是这些小型反刍动物非典型寄生虫的 物种。为此，检查了47只家绵羊和9只山羊的肌肉样本。使用针对 的直接PCR和巢式PCR以及对阳性扩增产物进行测序来鉴定 物种。除了在各自的中间宿主中检测到典型的 spp. 外，在绵羊中还检测到了 . 和 . 的DNA，尽管这些物种以前分别被认为是山羊和鹿特有的。此外，在山羊中发现了来自 . 和 . 的DNA，尽管这两个物种被认为是绵羊特有的。值得注意的是，在光学显微镜下，仅观察到山羊特有的 . 的肉孢子虫。因此，有必要对所检查的 spp. 的生命周期和宿主特异性进行进一步研究。
