Centre of Region Haná for Biotechnological and Agricultural Research, Czech Advanced Technology and Research Institute, Palacky University, Šlechtitelů 27, 78371 Olomouc, Czech Republic; Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute, Šlechtitelů 29, 78371 Olomouc, Czech Republic.
Centre of Region Haná for Biotechnological and Agricultural Research, Czech Advanced Technology and Research Institute, Palacky University, Šlechtitelů 27, 78371 Olomouc, Czech Republic.
Food Chem. 2022 May 1;375:131845. doi: 10.1016/j.foodchem.2021.131845. Epub 2021 Dec 13.
This work presents a pipeline for optimizing semi-hydroponic growth conditions and analyzes the impact on the growth and metabolism in three Mentha species (M. arvensis, M. x piperita, or M. spicata) and three Ocimum basilicum genotypes (́Chládek červená́, ́Litrá, or ́Máneś). The plant growth and the content- of nitrogen-containing compounds, phenolics, and terpenoids were determined under different nitrate concentrations and salt stress. Different responses were observed among genotypes for both species. ́Chládek červená́ had the best growth under low nitrate, with lower histamine and higher flavonoid levels. Mentha x piperita was the best mint species performing under low nitrate and salt stress. Altogether, we demonstrate that a combination of phenomics and metabolomics is ideal to identify the optimal growth conditions for these plants and the chemical markers associated with these conditions. Besides, we showed that both primary and secondary metabolites can be good markers for classifying both species.
本工作提出了一个优化半水培生长条件的流程，并分析了不同硝酸根浓度和盐胁迫条件对 3 种薄荷属（M. arvensis、M. x piperita 或 M. spicata）和 3 种罗勒属（́Chládek červená́、́Litrá 或 ́Máneś）植物生长和含氮化合物、酚类和萜类物质代谢的影响。在不同的硝酸根浓度和盐胁迫下，测定了植物的生长和含氮化合物、酚类和萜类物质的含量。不同基因型的两种植物表现出不同的反应。́Chládek červená́在低硝酸根条件下生长最好，组胺含量较低，类黄酮含量较高。M. x piperita 在低硝酸根和盐胁迫下是表现最好的薄荷属植物。总之，我们证明了表型组学和代谢组学的结合是确定这些植物最佳生长条件和与这些条件相关的化学标记物的理想方法。此外，我们还表明，初生代谢物和次生代谢物都可以作为分类这两个物种的良好标记物。
