Department of Neurosurgery, Shenzhen University General Hospital, Xueyuan AVE 1098, Nanshan District, Shenzhen, Guangdong,China.
Bashkir State Medical University, Lenina str., 3, 450008, Ufa,Russian Federation.
Curr Neurovasc Res. 2021;18(5):525-534. doi: 10.2174/1567202619666211217142116.
Intracranial aneurysms (IAs) are characterized by abnormal dilation and thinning of the cerebral vessels wall, leading to rupture and life-threatening aneurysmal subarachnoid hemorrhage (aSAH) condition. This dictates the need to find new biomarkers that predict the presence of IAs and the risk of their rupture. The aim of this study was to measure circulating miR-126 at various time points post-aSAH to identify the timing of peak levels.
Plasma samples from 62 patients with unruptured IAs (UIAs), 80 patients with aSAH at various time points (1, 3, 7, and 14 days post-event), and 47 healthy control were collected and subjected to qRT-PCR analyses for the expression levels of circulating miR-126. ROC curve and AUC were used to evaluate the diagnostic value of circulating miR-126.
The expression levels of circulating miR-126 were increased in patients with UIAs than in the healthy control. Furthermore, the expression levels of circulating miR-126 rose substantially from day 1 to day 7, but with a moderate decrease from day 7 to day 14 in plasma of patients with aSAH. The peak was observed on day 7. The AUC for miR-126 was 0.75, 0.75, 0.82, 0.87, and 0.79, respectively, and demonstrated that circulating miR-126 displayed considerable accuracy in discriminating plasma of patients with UIAs and patients after aSAH at various time points from a healthy control.
Our results indicated that circulating miR-126 in plasma samples could be served as a potential non-invasive biomarker in IAs detection and prevention IAs with a high risk of rupture.
颅内动脉瘤（IA）的特征是脑血管壁异常扩张和变薄，导致破裂和危及生命的蛛网膜下腔出血（aSAH）。这就需要寻找新的生物标志物来预测 IA 的存在和破裂的风险。本研究的目的是测量 aSAH 后不同时间点的循环 miR-126，以确定峰值时间。
收集 62 例未破裂颅内动脉瘤（UIAs）患者、80 例不同时间点（aSAH 后 1、3、7 和 14 天）的 aSAH 患者和 47 例健康对照者的血浆样本，并进行 qRT-PCR 分析循环 miR-126 的表达水平。ROC 曲线和 AUC 用于评估循环 miR-126 的诊断价值。
UIAs 患者的循环 miR-126 表达水平高于健康对照组。此外，循环 miR-126 的表达水平从第 1 天到第 7 天显著升高，但在 aSAH 患者的血浆中从第 7 天到第 14 天中度下降。第 7 天出现峰值。miR-126 的 AUC 分别为 0.75、0.75、0.82、0.87 和 0.79，表明循环 miR-126 在区分 UIAs 患者和不同时间点 aSAH 患者与健康对照组的血浆方面具有相当的准确性。
我们的结果表明，血浆样本中的循环 miR-126 可以作为一种潜在的非侵入性生物标志物，用于检测 IA 和预防具有高破裂风险的 IA。
