Liu Mengshi, Wang Kanghui, Chen Baizhong, Cai Yi, Li Chuwen, Yang Wanling, Wei Minyan, Zheng Guodong
Key Laboratory of Molecular Target & Clinical Pharmacology and the State Key Laboratory of Respiratory Disease & The Fifth Affiliated Hospital, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou 511436, China.
Guangdong Xinbaotang Biological Technology Co., Ltd, Guangdong, Jiangmen 529000, China.
Evid Based Complement Alternat Med. 2021 Dec 9;2021:2609935. doi: 10.1155/2021/2609935. eCollection 2021.
Citri Reticulatae Pericarpium, the desiccative mature peel of Blanco or its cultivated varieties, is a national geographical indicated product that has the concomitant function of both medicine and foodstuff. The primary source of Citri Reticulatae Pericarpium is "Chachi," called "Guang chenpi," while it differs in variety, propagation, grafting rootstock, and tree age, and the hereditary stability of its biological information between intraspecific plants is worthy of our attention. Homologous analysis result of 4 DNA barcodings in the ribosome or the chloroplast showed that the homology of them (ITS2, rbcl, matK, and psbA-trnH) of 22 samples was 100.00%, 99.97%, 99.99%, and 99.81%, respectively, which indicated that 4 DNA barcodes maintained a high degree of genetic stability in "Chachi." Also, ITS2 was considered to identify "Chachi" from other varieties because it presented not only low variability within a certain taxon but also a high level of interspecies variability. Simultaneously, variant site detection of "Chachi" was analyzed by comparing with the reference genome, and 2652697 SNP sites and 533906 InDel sites were detected from whole-genome resequencing data of 22 samples, providing the data resources and theoretical foundation for the future study about the relevant molecular makers of "Guang chenpi."
陈皮为芸香科植物橘及其栽培变种的干燥成熟果皮，是一种具有药食同源功能的国家地理标志产品。陈皮的主要来源为“茶枝柑”，又称“广陈皮”，其品种、繁殖方式、嫁接砧木和树龄不同，种内植物间生物信息的遗传稳定性值得关注。对核糖体或叶绿体中的4种DNA条形码进行同源性分析，结果显示22个样品的ITS2、rbcl、matK和psbA-trnH的同源性分别为100.00%、99.97%、99.99%和99.81%，表明这4种DNA条形码在“茶枝柑”中保持了高度的遗传稳定性。此外，ITS2被认为可用于区分“茶枝柑”与其他品种，因为它不仅在特定分类群内具有低变异性，而且在种间具有高变异性。同时，通过与参考基因组比较，对“茶枝柑”的变异位点进行检测，从22个样品的全基因组重测序数据中检测到2652697个SNP位点和533906个InDel位点，为今后“广陈皮”相关分子标记的研究提供了数据资源和理论基础。
