School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Chin J Nat Med. 2019 Oct;17(10):738-745. doi: 10.1016/S1875-5364(19)30090-1.
Herba Anoectochili is a commonly used medicinal material. However, its adulteration is a serious concern. Due to the similar morphological characteristics of Herba Anoectochili and its adulterants, traditional identification techniques often fail to distinguish between them accurately, which is not conducive to the circulation management and safety of the medicinal materials. To improve the distinction between Herba Anoectochili and its adulterants accurately, this study identified 41 Herba Anoectochili and its adulterant samples based on the ITS2 sequence. Sequence characteristics, Basic Local Alignment Search Tool (BLAST) application, genetic distance, construction of phylogenetic tree, secondary structure prediction, and other methods showed the ITS2 sequence to accurately identify Herba Anoectochili from its adulterants. Furthermore, in this study, we designed a specific primer, based on the ITS2 sequence, and established a real-time PCR detection system for the rapid, sensitive, and specific identification of the original plant of Herba Anoectochili. Compared to DNA barcoding technology, this method has shorter detection time, stronger specificity, and higher sensitivity, which lays the foundation for the rapid identification of Herba Anoectochili.
龙牙草是一种常用的药材。然而，其掺伪问题令人担忧。由于龙牙草及其掺伪品在形态特征上具有相似性，传统的鉴定技术往往无法准确区分，这不利于药材的流通管理和使用安全。为了提高龙牙草及其掺伪品的鉴别准确性，本研究基于 ITS2 序列对 41 份龙牙草及其掺伪品样本进行了鉴定。序列特征、BLAST 应用、遗传距离、系统发育树构建、二级结构预测等方法表明，ITS2 序列能够准确地鉴别龙牙草与其掺伪品。此外，本研究还基于 ITS2 序列设计了一对特异性引物，并建立了一种实时 PCR 检测系统，用于快速、灵敏、特异性地鉴定龙牙草的原植物。与 DNA 条形码技术相比，该方法检测时间更短，特异性更强，灵敏度更高，为龙牙草的快速鉴定奠定了基础。
