Philip A, Carter-Scott P, Rogers C
J Infect Dis. 1987 Feb;155(2):304-8. doi: 10.1093/infdis/155.2.304.
In subjects with trichomoniasis the number of Trichomonas vaginalis in vaginal discharges or secretions is unknown. The presence of T. vaginalis was evaluated in 177 consecutive female patients attending an inner city sexually transmitted disease clinic by patient history, wet mount, and broth culture. T. vaginalis was quantitated by a novel agar culture technique. Of the 177 women, 86 (49%) were positive for T. vaginalis by either wet mount or culture. Clinical symptoms were not reliable for making an accurate diagnosis of trichomoniasis. Culture on modified Diamond's medium was more sensitive (98%) than the wet mount method (38%) in detecting T. vaginalis. Of the 86 patients who were positive for trichomoniasis, quantitation was obtained for 81 patients, with 70% yielding greater than 10(4) colony-forming units (cfu)/ml. The number of T. vaginalis ranged from 40 to greater than 10(6) cfu/ml. The wet mount method was very insensitive for detecting T. vaginalis and was positive only in patients yielding greater than 10(5) cfu/ml.
在患有滴虫病的患者中，阴道分泌物或排泄物中阴道毛滴虫的数量尚不清楚。通过病史、湿片检查和肉汤培养，对一家市中心性传播疾病诊所连续就诊的177名女性患者进行了阴道毛滴虫检测。采用一种新型琼脂培养技术对阴道毛滴虫进行定量。在这177名女性中，86名（49%）通过湿片检查或培养确诊为阴道毛滴虫阳性。临床症状对于准确诊断滴虫病并不可靠。改良钻石培养基培养法在检测阴道毛滴虫方面比湿片检查法（38%）更敏感（98%）。在86名滴虫病阳性患者中，对81名患者进行了定量检测，其中70%的患者每毫升产生超过10⁴个菌落形成单位（cfu）。阴道毛滴虫数量范围为每毫升40至超过10⁶个cfu。湿片检查法在检测阴道毛滴虫方面非常不敏感，仅在每毫升产生超过10⁵个cfu的患者中呈阳性。
