Synovial mesenchymal stem cell-derived exosomal enhances chondrogenesis by targeting ADAM19.

Synovial mesenchymal stem cell (SMSC)-derived exosomes show treatment potential in osteoarthritis, although their functional mechanism is still unclear. Osteoarthritis chondrocytes and normal SMSC were cultured. Subsequently, chondrocytes were co-cultured with SMSC or -overexpressing SMSC-derived exosomes, or directly transfected with mimic. Furthermore, compensate experiments were conducted. SMSC promoted chondrocyte proliferation, migration, COL2A1 and ACAN expressions while suppressing apoptosis by transmitting exosomes. Furthermore, -overexpressing SMSC-derived exosomes and direct overexpression in chondrocytes presented more significant effect on enhancing chondrogenesis. In addition, directly targeted ADAM19, and ADAM19 overexpression compensated the regulation of on chondrogenesis. SMSC-derived exosomal enhances chondrogenesis through targeting ADAM19, highlighting a potentially novel mechanism of SMSC in treating osteoarthritis.