National Engineering Research Centre for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medical, Huazhong University of Science and Technology, Wuhan 430074, China.
Chem Soc Rev. 2022 Jan 24;51(2):566-593. doi: 10.1039/d1cs00525a.
The real-time, dynamic optical visualization of lesions and margins ensures not only complete resection of the malignant tissues but also better preservation of the vital organs/tissues during surgical procedures. Most imaging probes with an "always-on" signal encounter high background noise due to their non-specific accumulation in normal tissues. By contrast, activatable molecular probes only "turn on" their signals upon reaction with the targeted biomolecules that are overexpressed in malignant cells, offering high target-to-background ratios with high specificity and sensitivity. This review summarizes the recent progress of activatable molecular probes in surgical imaging and diagnosis. The design principle and mechanism of activatable molecular probes are discussed, followed by specific emphasis on applications ranging from fluorescence-guided surgery to endoscopy and tissue biopsy. Finally, potential challenges and perspectives in the field of activatable molecular probe-enabled surgical imaging are discussed.
实时动态光学可视化病变和边界不仅确保恶性组织的完全切除，而且在手术过程中更好地保护重要器官/组织。由于大多数具有“常开”信号的成像探针在正常组织中非特异性积累，因此会遇到高背景噪声。相比之下，活化分子探针仅在与恶性细胞中过度表达的靶向生物分子反应时才“打开”其信号，从而提供高靶标与背景的比值，具有高特异性和灵敏度。这篇综述总结了可活化分子探针在手术成像和诊断中的最新进展。讨论了可活化分子探针的设计原理和机制，并特别强调了从荧光引导手术到内窥镜检查和组织活检的应用。最后，讨论了可活化分子探针在手术成像领域面临的潜在挑战和前景。
