Department of Immunology and Allergology, Lithuanian University of Health Sciences, Eiveniu str. 2, Kaunas, Lithuania.
Laboratory of Immunology, Department of Immunology and Allergology, Lithuanian University of Health Sciences, Eiveniu str. 2, 50009, Kaunas, Lithuania.
BMC Pulm Med. 2021 Dec 21;21(1):424. doi: 10.1186/s12890-021-01798-8.
To evaluate cytokine profile, vitamin D status, symptom score and quality of life in patients with persistent allergic airway diseases sensitised to house dust mites (HDM) in comparison with healthy individuals.
Patients sensitized to HDM with persistent AR and having symptoms for at least 2 years with or without AA were involved into the study. Measurements of vitamin D level in serum and IL-10, IL-13, IL-17, IL-22, IL-33 and IFN-gamma in serum and nasal lavage were performed by ELISA.
Eighty-one subjects were involved into the study. Serum IL-10 concentration was higher in patients with AR than in patients with AR and AA (6.71 ± 1.73 vs. 1.98 ± 0.24, p < 0.05). IFN-gamma level in nasal lavage was higher in patients with AR and AA than in patients with AR (p < 0.01) and healthy individuals (p < 0.05) (7.50 ± 0.37 vs. 6.80 ± 0.99 vs. 6.50 ± 0.22). Serum IL-22 negatively correlated with IL-22 in nasal lavage, whereas serum IFN-gamma positively correlated with IFN-gamma in nasal lavage. Positive correlation between serum IL-17 and total IgE and negative correlation between IL-17 in nasal lavage and eosinophils in nasal smear were found in patients with AR and AA. Serum IFN-gamma decreased the risk of AR for healthy individuals. Serum IL-10 and vitamin D decreased risk for development of AA for patients with AR. IL-22 in serum and IL-10 and IL-33 in nasal lavage increased this risk.
Novel cytokines such as IL-22, IL-17 and IL-33 and vitamin D may be involved in pathogenesis of persistent airway inflammation in patients sensitized to HDM.
评估对屋尘螨（HDM）过敏的持续性过敏性气道疾病患者的细胞因子谱、维生素 D 状态、症状评分和生活质量，并与健康个体进行比较。
本研究纳入了对 HDM 过敏、持续性 AR 且有至少 2 年症状的患者，无论是否伴有 AA。通过 ELISA 法测定血清中维生素 D 水平及血清和鼻洗液中 IL-10、IL-13、IL-17、IL-22、IL-33 和 IFN-γ的水平。
共有 81 名受试者参与了研究。与 AR 患者相比，AR 和 AA 患者的血清 IL-10 浓度更高（6.71±1.73 比 1.98±0.24，p<0.05）。AR 和 AA 患者鼻洗液中 IFN-γ水平高于 AR 患者（p<0.01）和健康个体（p<0.05）（7.50±0.37 比 6.80±0.99 比 6.50±0.22）。血清 IL-22 与鼻洗液中的 IL-22 呈负相关，而血清 IFN-γ与鼻洗液中的 IFN-γ呈正相关。在 AR 和 AA 患者中，血清 IL-17 与总 IgE 呈正相关，鼻洗液中 IL-17 与鼻黏膜嗜酸性粒细胞呈负相关。血清 IFN-γ降低了健康个体患 AR 的风险。血清 IL-10 和维生素 D 降低了 AR 患者发生 AA 的风险。血清 IL-22 和鼻洗液中 IL-10 和 IL-33 增加了这种风险。
新型细胞因子如 IL-22、IL-17 和 IL-33 以及维生素 D 可能参与了对 HDM 过敏的持续性气道炎症的发病机制。
