Department of Pesticide Science, College of Plant Protection, Shenyang Agricultural University, 120 Dongling Road, Shenhe District, Shenyang City, Liaoning Province, 110000, China.
Department of Pesticide Science, College of Plant Protection, Shenyang Agricultural University, 120 Dongling Road, Shenhe District, Shenyang City, Liaoning Province, 110000, China.
Fungal Biol. 2022 Jan;126(1):47-53. doi: 10.1016/j.funbio.2021.10.002. Epub 2021 Oct 19.
Azoxystrobin has been widely used since 1996 to control rice blast caused by Pyricularia oryzae. Azoxystrobin resistance related to mutations at the P. oryzae target protein (F129L of Cytb) has been reported worldwide. To quickly identify and detect resistant strains in the field, this research established a rapid loop-mediated isothermal amplification (LAMP) detection system for the F129L mutation. The system could detect the P. oryzae F129L (TTC-TTA) mutation at 62 °C within 60 min, with a detection limit of 100 fg/μL, which is 10 times higher than for conventional PCR. The method had high specificity and repeatability and could detect the F129L (TTC-TTA) mutation in plant tissues within 3 h. The LAMP method established in this study will be useful to detect azoxystrobin-resistant P. oryzae F129L mutant strains and generate significant data for the management of resistant P. oryzae isolates.
唑菌酯自 1996 年以来被广泛用于防治稻瘟病菌引起的稻瘟病。全球范围内已报道唑菌酯抗性与靶标蛋白（细胞色素 b 中的 F129L）突变有关。为了在田间快速鉴定和检测抗性菌株，本研究建立了一种针对 F129L 突变的快速环介导等温扩增（LAMP）检测系统。该系统可在 62°C 下 60 分钟内检测到稻瘟病菌 F129L（TTC-TTA）突变，检测限为 100 fg/μL，比常规 PCR 高 10 倍。该方法具有高度特异性和重复性，可在 3 小时内检测到植物组织中的 F129L（TTC-TTA）突变。本研究建立的 LAMP 方法将有助于检测唑菌酯抗性稻瘟病菌 F129L 突变株，并为稻瘟病菌抗性分离物的管理提供有意义的数据。
