Wang Yinan, Yang Di, Bi Yuhui, Yu Zhiguo
College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China.
Engineering & Technological Research Center of Biopesticide for Liaoning Province, Shenyang 110866, China.
Microorganisms. 2022 Aug 9;10(8):1612. doi: 10.3390/microorganisms10081612.
causes rice blast, the major destructive disease in nearly all rice fields. In order to obtain highly active compounds against , four new 20-membered macrolides named venturicidins G-J () were isolated from the culture broth of sp. SN5452 along with two known ones, venturicidins A () and B (). Their structures were determined by the cumulative analyses of nuclear magnetic resonance (NMR) spectroscopy and high-resolution electrospray ionization mass spectrometry (HRESIMS) data. All isolated compounds were evaluated for their antifungal activity against . Interestingly, these compounds exhibited obvious inhibition to mycelial growth and conidial germination of . Remarkably, the EC values of venturicidins A (), B (), and I () against mycelial growth were 0.11, 0.15 and 0.35 µg/mL, and their EC values of conidial germination were 0.27, 0.39 and 1.14 µg/mL, respectively. The analysis of structure-activity relationships (SARs) revealed that the methylated positions might be involved in the antifungal activity of venturicidins. These results indicate that the venturicidins are prospective candidates for novel fungicides that can be applied in controlling rice blast.
引起稻瘟病，这是几乎所有稻田中的主要毁灭性病害。为了获得对[病害名称未明确]具有高活性的化合物，从[菌株名称未明确]sp. SN5452的培养液中分离出四种名为venturicidins G - J（[具体结构未给出]）的新的20元大环内酯类化合物以及两种已知的venturicidins A（[具体结构未给出]）和B（[具体结构未给出]）。它们的结构通过核磁共振（NMR）光谱和高分辨率电喷雾电离质谱（HRESIMS）数据的累积分析得以确定。对所有分离出的化合物进行了针对[病害名称未明确]的抗真菌活性评估。有趣的是，这些化合物对[病害名称未明确]的菌丝生长和分生孢子萌发表现出明显的抑制作用。值得注意的是，venturicidins A（[具体结构未给出]）、B（[具体结构未给出]）和I（[具体结构未给出]）对菌丝生长的EC值分别为0.11、0.15和0.35 µg/mL，它们对分生孢子萌发的EC值分别为0.27、0.39和1.14 µg/mL。构效关系（SARs）分析表明，甲基化位置可能与venturicidins的抗真菌活性有关。这些结果表明，venturicidins是可用于防治稻瘟病的新型杀菌剂的潜在候选物。
