Chair and Department of Cell Biology, Faculty of Health Sciences, Poznan University of Medical Sciences, Rokietnicka 5D, 60-806, Poznan, Poland.
Institute of Microecology, Sielska 6, 60-129, Poznan, Poland.
Sci Rep. 2021 Dec 20;11(1):24274. doi: 10.1038/s41598-021-03794-8.
The early-life modifications of intestinal microbiota may impact children's subsequent emotional and cognitive development. Studies show that some bacteria species in gut microbiota, and the lack of others, may play a key role in autism spectrum disorders (ASD) development. Fecal samples were obtained from three groups of children: 16 healthy, 24 with allergies (ALG), and 33 with ASD (probiotics and non-probiotics users). The analysis was carried out according to the KyberKompakt Pro protocol. We observed a significantly higher level of Klebsiella spp. in the healthy children from the non-probiotics group, considering three groups. In the same group, Bifidobacterium spp. the level was lower in ASD compared to neurotypical individuals. In healthy children who did not use probiotics, strong positive correlations were observed in E. coli and Enterococcus spp. and Bacteroides and Klebsiella spp., and a negative correlation for Akkermansia muciniphila with both Klebsiella spp. and Bacteroides spp. In the ASD group who take probiotics, a strongly negative correlation was observed in Lactobacillus spp., and both Faecalibacterium prausnitzii and Akkermansia muciniphila levels. In the ALG group, the strongest, negative correlation was found between Enterococcus spp. and Lactobacillus spp. as in Akkermansia muciniphila and Bifidobacterium spp. The simple commercial test revealed minor differences in the composition of intestinal microorganisms between children with autism spectrum disorders and neurotypical peers.
早期肠道微生物群的改变可能会影响儿童随后的情绪和认知发展。研究表明，肠道微生物群中的一些细菌种类，以及其他细菌种类的缺乏，可能在自闭症谱系障碍（ASD）的发展中起关键作用。从三组儿童中获得粪便样本：16 名健康儿童、24 名过敏儿童（ALG）和 33 名 ASD 儿童（益生菌和非益生菌使用者）。根据 KyberKompakt Pro 方案进行分析。我们观察到，在非益生菌组的健康儿童中，克雷伯氏菌属的水平明显更高，而在同一组中，双歧杆菌属的水平在 ASD 儿童中比神经典型个体更低。在未使用益生菌的健康儿童中，大肠杆菌和肠球菌属与拟杆菌属和克雷伯氏菌属之间存在强烈的正相关，而阿克曼氏菌属与克雷伯氏菌属和拟杆菌属之间存在负相关。在使用益生菌的 ASD 组中，乳酸杆菌属和 both Faecalibacterium prausnitzii 和阿克曼氏菌属的水平呈强烈负相关。在 ALG 组中，肠球菌属与乳酸杆菌属之间存在最强的负相关，与阿克曼氏菌属和双歧杆菌属之间也存在负相关。简单的商业测试揭示了自闭症谱系障碍儿童与神经典型同龄人之间肠道微生物组成的微小差异。
