Pereira-Caro Gema, Almutairi Tahani M, Crozier Alan, Ordoñez-Díaz José Luis, Moreno-Rojas José Manuel, Ortiz-Somovilla Víctor, Morillo-Santander Gabriela, Lazarova Svilena, Malkova Dalia, García Ada L
Department of Food Science and Health, IFAPA-Alameda del Obispo, Córdoba, Spain.
Department of Chemistry, King Saud University, Riyadh, Saudi Arabia.
Int J Food Sci Nutr. 2022 Jun;73(4):531-537. doi: 10.1080/09637486.2021.2015572. Epub 2021 Dec 21.
The impact of β-glucan on the bioavailability of orange juice (OJ) flavanones was investigated in a randomised controlled trial. Volunteers consumed 500 mL of OJ without or with either 3 g (OB-3) or 6 g (OB-6) of β-glucan. Urine samples, collected 12 h before and over a 0-24 h period post-supplementation, were analysed by high-performance liquid chromatography-high resolution mass spectrometry. The overall 0-24 h urinary excretion of the 17 flavanone metabolites identified and quantified in urine after OJ ingestion corresponded to 29.7 µmol, and 25.0 and 9.3 µmol, respectively, after OB-3 and OB-6 intake. This corresponds to 9.3, 7.9, and 2.9% recoveries of the 318 µmol of the ingested flavanones. The acute ingestion of OJ with 6 g, but not 3 g of β-glucan led to a significant reduction ( < 0.05) in the excretion of flavanone metabolites compared with consumption of OJ alone.
在一项随机对照试验中，研究了β-葡聚糖对橙汁（OJ）黄酮类化合物生物利用度的影响。志愿者饮用500毫升不含β-葡聚糖的橙汁，或分别添加3克（OB-3）或6克（OB-6）β-葡聚糖的橙汁。在补充前12小时以及补充后0至24小时内收集尿液样本，采用高效液相色谱-高分辨率质谱法进行分析。摄入橙汁后，尿液中鉴定并定量的17种黄酮类代谢物的0至24小时总尿排泄量为29.7微摩尔，摄入OB-3和OB-6后分别为25.0微摩尔和9.3微摩尔。这相当于摄入的318微摩尔黄酮类化合物的回收率分别为9.3%、7.9%和2.9%。与单独饮用橙汁相比，急性摄入添加6克而非3克β-葡聚糖的橙汁导致黄酮类代谢物排泄量显著降低（<0.05）。
