Tan Yusheng, Agustin Roman Vincent C, Stein Lisa Y, Sauvageau Dominic
Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, Canada.
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.
Biotechnol Rep (Amst). 2021 Dec 3;32:e00691. doi: 10.1016/j.btre.2021.e00691. eCollection 2021 Dec.
Industrial fermentation provides a wide variety of bioproducts, such as food, biofuels and pharmaceuticals. Self-cycling fermentation (SCF), an advanced automated semi-continuous fermentation approach, has shown significant advantages over batch reactors (BR); including cell synchrony and improved production. Here, engineered to overproduce shikimic acid was grown under SCF operation. This led to four-fold increases in product yield and volumetric productivity compared to BR. Transcriptomic analyses were performed to understand the cellular mechanisms leading to these increases. Results indicate an up-regulation of a large number of genes related to the cell cycle and DNA replication in the early stages of SCF cycles, inferring substantial synchronization. Moreover, numerous genes related to gluconeogenesis, the citrate cycle and oxidative phosphorylation were significantly up-regulated in the late stages of SCF cycles, consistent with significant increases in shikimic acid yield and productivity.
工业发酵可提供多种生物产品，如食品、生物燃料和药品。自循环发酵（SCF）是一种先进的自动化半连续发酵方法，已显示出相对于分批反应器（BR）的显著优势，包括细胞同步性和产量提高。在此，对经过工程改造以过量生产莽草酸的菌株进行了SCF操作培养。与分批反应器相比，这使得产物产量和体积生产率提高了四倍。进行了转录组分析以了解导致这些增加的细胞机制。结果表明，在SCF循环的早期阶段，大量与细胞周期和DNA复制相关的基因上调，这意味着显著的同步性。此外，在SCF循环的后期阶段，许多与糖异生、柠檬酸循环和氧化磷酸化相关的基因显著上调，这与莽草酸产量和生产率的显著增加一致。
