Sasaki M, Kato S, Kohno K, Martin G R, Yamada Y
Proc Natl Acad Sci U S A. 1987 Feb;84(4):935-9. doi: 10.1073/pnas.84.4.935.
Laminin is a basement membrane-specific glycoprotein (800 kDa) consisting of three chains: A, B1, and B2. Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. We have isolated two overlapping cDNA clones that span 5.9 kilobases and code for the entire B1 chain of mouse laminin. The nucleotide sequence of the clones reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. Analysis of the deduced protein sequence predicts that the B1 chain has seven distinct domains that include cysteine-rich repeats, alpha-helical, and globular structures. Part of the cysteine-rich region is homologous to epidermal growth factor and other proteins that contain epidermal growth factor-like repeats.
层粘连蛋白是一种基底膜特异性糖蛋白（800 kDa），由三条链组成：A链、B1链和B2链。层粘连蛋白具有多种生物学功能，包括刺激上皮细胞生长和分化。我们分离出了两个重叠的cDNA克隆，它们跨度为5.9千碱基，编码小鼠层粘连蛋白的整个B1链。这些克隆的核苷酸序列揭示了一个5358个碱基对的开放阅读框，可能编码1786个氨基酸，包括一个推定信号肽的20个氨基酸。对推导的蛋白质序列的分析预测，B1链有七个不同的结构域，包括富含半胱氨酸的重复序列、α-螺旋和球状结构。富含半胱氨酸区域的一部分与表皮生长因子以及其他含有表皮生长因子样重复序列的蛋白质同源。
