Facultad de Medicina Veterinaria y Zootecnia "Dr. Norberto Treviño Zapata", Universidad Autónoma de Tamaulipas, Carretera Victoria-Mante km 5.5, 87000, Ciudad Victoria, TAMPS, México.
Departamento de Ecología de Agentes Patógenos, Hospital General "Dr. Manuel Gea González", Ciudad de México, México.
Arch Microbiol. 2021 Dec 22;204(1):52. doi: 10.1007/s00203-021-02664-1.
The invA gene of Brucella melitensis codes for a NUDIX (nucleoside diphosphate linked to moiety X) hydrolase related to invasiveness. The objective of this work was to evaluate invA transcription under acidic conditions. The invA gene transcription was up regulated at pH 3 and pH 5 observed with semiquantitative real-time PCR in B. melitensis 133 strain. Results indicated that invA gene transcription at pH 3 showed a basal and decreased transcription compared to that of pH 5 incubation. Transcription levels of the dnaK gene were similar to those obtained with invA gene. The survival rates of wild type and invA mutant strains at pH 5 were above 90% in all post-incubation times. In contrast, at pH 3 there was a time-dependent reduction on both strains at 15 min (P < 0.05). These results suggest that invA gene transcription is promoted under acidic conditions in Brucella melitensis.
绵羊布鲁氏菌的 invA 基因编码一种与侵袭性相关的 NUDIX（核苷二磷酸连接到 X 部分）水解酶。本工作的目的是评估酸性条件下 invA 的转录情况。通过半定量实时 PCR 观察到在 pH 3 和 pH 5 时，绵羊布鲁氏菌 133 株的 invA 基因转录上调。结果表明，与 pH 5 孵育相比，pH 3 时 invA 基因的转录呈现基础和降低的转录。dnaK 基因的转录水平与 invA 基因相似。在所有孵育时间后，野生型和 invA 突变株在 pH 5 下的存活率均高于 90%。相比之下，在 pH 3 下，两种菌株在 15 分钟时均呈时间依赖性降低（P<0.05）。这些结果表明，在绵羊布鲁氏菌中，invA 基因的转录在酸性条件下被促进。
