Lovett D H, Haensch G M, Goppelt M, Resch K, Gemsa D
J Immunol. 1987 Apr 15;138(8):2473-80.
Treatment of cultured renal glomerular mesangial cells (MC) with nonlytic concentrations of the purified components (C5b-9) of the terminal membrane attack complex (MAC) of complement induced significant functional alterations characteristic of cellular activation. C5b-9-treated MC released large quantities of primarily vasodilatory prostaglandins. In addition, the secretion of an MC-derived auto-growth factor (MC interleukin 1) was greatly enhanced. Examination of the action of C5b-9 on MC phospholipid metabolism indicated that complement induced the activation of phospholipases, leading to quantitative changes in the fatty acid profile of MC membrane phospholipids. These findings demonstrate that cultured MC are highly responsive to nonlytic concentrations of the C5b-9 complex, and suggest that the mesangial deposition of the MAC in many forms of glomerular disease, with resultant cellular activation, may play a major role in the hemodynamic and cellular proliferative events characteristic of these disorders.
用补体终末膜攻击复合物（MAC）的纯化成分（C5b-9）的非溶解浓度处理培养的肾小球系膜细胞（MC），可诱导出具有细胞活化特征的显著功能改变。经C5b-9处理的MC释放大量主要为血管舒张性的前列腺素。此外，MC衍生的自生长因子（MC白细胞介素1）的分泌也大大增强。对C5b-9对MC磷脂代谢作用的研究表明，补体可诱导磷脂酶活化，导致MC膜磷脂脂肪酸谱的定量变化。这些发现表明，培养的MC对C5b-9复合物的非溶解浓度高度敏感，并提示MAC在多种形式的肾小球疾病中的系膜沉积以及由此导致的细胞活化，可能在这些疾病特征性的血流动力学和细胞增殖事件中起主要作用。
