Yunnan Institute of Microbiology, Key Laboratory for Conservation and Utilization of Bio-Resource, and Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming, 650091, People's Republic of China.
Arch Microbiol. 2021 Dec 23;204(1):60. doi: 10.1007/s00203-021-02676-x.
A Gram stain-positive, rod-shaped, and subterminal endospore-forming bacterium, designated strain YIM B01967, was isolated from a forest soil sample collected in Ailaoshan National Nature Reserve, Yuxi City, Xinpin county, Yunnan province, China. Strain YIM B01967 showed the highest 16S rRNA gene sequence similarity with Viridibacillus arvi (99.1%) and Viridibacillus arenosi (98.9%). Based on the phylogenetic and 16S rRNA gene sequence results, strain YIM B01967 was affiliated to the genus Viridibacillus. The growth of YIM B01967 was observed at 15-35 °C (optimum, 28 °C), pH 7.0-9.0 (optimum, pH 7.5) and in the presence of 0-2% (w/v) NaCl (optimum in 2% NaCl). The cell wall sugars include ribose, glucose, arabinose, galactose, and mannose. The quinone system consisted of the major compound MK-8 and moderate amounts of MK-7. The major fatty acids (> 10%) included iso-C, anteiso-C, C ω10c. The major polar lipids profile included DPG, PME. The cell wall peptidoglycan was most likely of the type A4α with an L-Lys-D-Asp interpeptide bridge. The genomic DNA G + C content of strain YIM B01967 was 36.3 mol%. The ANI and digital DNA-DNA hybridization (dDDH) values between strain YIM B01967 and Viridibacillus arvi DSM 16317, Viridibacillus arenosi DSM 16319 were 61.0% and 32.1%, 60.0% and 33.1% based on the draft genome sequence. The results support the conclusion that strain YIM B01967 represents a novel species of the genus Viridibacillus, for which the name Viridibacillus soli sp. nov., is proposed. The type strain is YIM B01967 (= KCTC 43249 = CGMCC 1.18436).
一株革兰氏阳性、杆状、末端内生孢子形成的细菌，被命名为 YIM B01967 菌株，从中国云南省玉溪市新平县哀牢山国家级自然保护区采集的森林土壤样本中分离得到。菌株 YIM B01967 与 Viridibacillus arvi（99.1%）和 Viridibacillus arenosi（98.9%）的 16S rRNA 基因序列相似度最高。基于系统发育和 16S rRNA 基因序列结果，菌株 YIM B01967 属于 Viridibacillus 属。YIM B01967 的生长温度为 15-35°C（最佳温度为 28°C），pH 值为 7.0-9.0（最佳 pH 值为 7.5），并且可以在 0-2%（w/v）NaCl 存在的情况下生长（在 2%NaCl 中生长最佳）。细胞壁糖包括核糖、葡萄糖、阿拉伯糖、半乳糖和甘露糖。醌类系统由主要化合物 MK-8 和适量的 MK-7 组成。主要脂肪酸（>10%）包括 iso-C、anteiso-C、C ω10c。主要极性脂谱包括 DPG、PME。细胞壁肽聚糖可能是 A4α 型，具有 L-Lys-D-Asp 间肽桥。菌株 YIM B01967 的基因组 DNA G+C 含量为 36.3 mol%。根据基因组草图序列，菌株 YIM B01967 与 Viridibacillus arvi DSM 16317、Viridibacillus arenosi DSM 16319 的 ANI 值和数字 DNA-DNA 杂交（dDDH）值分别为 61.0%和 32.1%、60.0%和 33.1%。这些结果支持以下结论：菌株 YIM B01967 代表了 Viridibacillus 属的一个新种，建议将其命名为 Viridibacillus soli sp. nov.。模式菌株为 YIM B01967（=KCTC 43249=CGMCC 1.18436）。
