Yunnan Institute of Microbiology, Key Laboratory for Southwest Microbial Diversity of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming, 650091, People's Republic of China.
Department of Medicine, Qujing Vocational and Technical College, Qujing, 655000, People's Republic of China.
Antonie Van Leeuwenhoek. 2024 Mar 1;117(1):48. doi: 10.1007/s10482-024-01942-4.
A gram-stain-positive, aerobic, rod-shaped bacterial strain capable of producing siderophores, named YIM B08730, was isolated from a soil sample collected from Wumeng Mountain National Nature Reserve, Zhaotong City, Yunnan Province. Growth occurred at 10-45 °C (optimum, 35-40 ℃), pH 7.0-9.0 (optimum, 7.0) and in the presence of 0-5 % (w/v) NaCl (optimum, 0-1 %, w/v). A comparative analysis of the 16S rRNA gene sequence (1558 bp) of strain YIM B08730 showed the highest similarity to Solibacillus isronensis JCM 13838 (96.2 %), followed by Solibacillus silvestris DSM 12223 (96.0 %) and Solibacillus kalamii ISSFR-015 (95.4 %). The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and one unidentified lipid. The main respiratory quinone of strain YIM B08730 was menaquinone 7 (MK-7). The major fatty acids were iso-C and Cω7c alcohol. The digital DNA-DNA hybridization and average nucleotide identity values between strain YIM B08730 and the reference strain S. isronensis JCM 13838 were 24.8 % and 81.2 %, respectively. The G + C content of the genomic DNA was 37.1 mol%. The genome of the novel strain contained genes associated with the production of siderophores, and it also revealed other functional gene clusters involved in plant growth promotion and soil bioremediation. Based on these phenotypic, chemotaxonomic and phylogenetic analyses, strain YIM B08730 is considered to be a novel species of the genus Solibacillus, for which the name Solibacillus ferritrahens sp. nov. is proposed. The type strain is YIM B08730 (= NBRC 116268 = CGMCC 1.60169).
一株能够产生铁载体的革兰氏阳性、需氧、杆状细菌菌株，命名为 YIM B08730，从云南省昭通市乌蒙山国家级自然保护区采集的土壤样本中分离得到。该菌在 10-45°C（最佳温度为 35-40°C）、pH7.0-9.0（最佳 pH 值为 7.0）和 0-5%（w/v）NaCl（最佳 0-1%，w/v）条件下生长。菌株 YIM B08730 的 16S rRNA 基因序列（1558 bp）的比较分析表明，其与 Solibacillus isronensis JCM 13838（96.2%）的相似度最高，其次是 Solibacillus silvestris DSM 12223（96.0%）和 Solibacillus kalamii ISSFR-015（95.4%）。主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰丝氨酸和一种未鉴定的脂。菌株 YIM B08730 的主要呼吸醌为menaquinone 7（MK-7）。主要脂肪酸为 iso-C 和 Cω7c 醇。菌株 YIM B08730 与参考菌株 Solibacillus isronensis JCM 13838 的数字 DNA-DNA 杂交和平均核苷酸同一性值分别为 24.8%和 81.2%。基因组 DNA 的 G+C 含量为 37.1mol%。新型菌株的基因组包含与铁载体产生相关的基因，还揭示了其他与植物生长促进和土壤生物修复相关的功能基因簇。基于这些表型、化学分类和系统发育分析，菌株 YIM B08730 被认为是 Solibacillus 属的一个新种，命名为 Solibacillus ferritrahens sp. nov.。模式菌株为 YIM B08730（=NBRC 116268=CGMCC 1.60169）。
