Gruntenko Nataly E, Karpova Evgenia K, Babenko Vladimir N, Vasiliev Gennady V, Andreenkova Olga V, Bobrovskikh Margarita A, Menshanov Petr N, Babenko Roman O, Rauschenbach Inga Yu
Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, Russia.
Physiology Department, Novosibirsk State University, 630090 Novosibirsk, Russia.
Biology (Basel). 2021 Dec 14;10(12):1323. doi: 10.3390/biology10121323.
Understanding how repeated stress affects metabolic and physiological functions in the long run is of crucial importance for evaluating anthropogenic pressure on the environment. We investigated fertility, longevity and metabolism in females exposed to short-term heat stress (38 °C, 1 h) repeated daily or weekly. Daily stress was shown to cause a significant decrease in both fertility and longevity, as well as in body mass and triglyceride (fat) content, but a significant increase in trehalose and glucose content. Weekly stress did not affect longevity and carbohydrate metabolism but resulted in a significant decrease in body mass and fat content. Weekly stress did not affect the total level of fertility, despite sharp fertility drops on the exact days of stressing. However, stressing insects weekly, only in the first two weeks after eclosion, caused a significant increase in the total level of fertility. The analysis of differentially expressed genes in the fat bodies and adjacent tissues of researched groups with the use of RNA-Seq profiling revealed changes in signal pathways related to proteolysis/digestion, heat shock protein 23, and in the tightly linked stress-inducible humoral factor gene network.
从长远来看，了解反复应激如何影响代谢和生理功能对于评估人为环境压力至关重要。我们研究了每天或每周重复暴露于短期热应激（38°C，1小时）的雌性昆虫的繁殖力、寿命和代谢情况。结果表明，每日应激会导致繁殖力和寿命显著下降，同时体重和甘油三酯（脂肪）含量也会显著降低，但海藻糖和葡萄糖含量会显著增加。每周应激对寿命和碳水化合物代谢没有影响，但会导致体重和脂肪含量显著下降。每周应激不会影响繁殖力的总体水平，尽管在应激的确切日子繁殖力会急剧下降。然而，仅在羽化后的前两周每周对昆虫进行应激处理，会导致繁殖力总体水平显著提高。利用RNA-Seq分析对研究组脂肪体和相邻组织中差异表达基因进行分析，结果显示与蛋白水解/消化、热休克蛋白23以及紧密相连的应激诱导体液因子基因网络相关的信号通路发生了变化。
