Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, Russia.
Int J Mol Sci. 2023 Dec 12;24(24):17411. doi: 10.3390/ijms242417411.
is a maternally inherited, intercellular bacterial symbiont of insects and some other invertebrates. Here, we investigated the effect of two different strains, differing in a large chromosomal inversion, on the differential expression of genes in females. We revealed significant changes in the transcriptome of the infected flies compared to the uninfected ones, as well as in the transcriptome of flies infected with the strain, wMelPlus, compared to flies infected with the wMelCS strain. We linked differentially expressed genes (DEGs) from two pairwise comparisons, "uninfected-wMelPlus-infected" and "uninfected-wMelCS-infected", into two gene networks, in which the following functional groups were designated: "Proteolysis", "Carbohydrate transport and metabolism", "Oxidation-reduction process", "Embryogenesis", "Transmembrane transport", "Response to stress" and "Alkaline phosphatases". Our data emphasized similarities and differences between infections by different strains under study: a wMelPlus infection results in more than double the number of upregulated DEGs and half the number of downregulated DEGs compared to a wMelCS infection. Thus, we demonstrated that made a significant contribution to differential expression of host genes and that the bacterial genotype plays a vital role in establishing the character of this contribution.
是一种母系遗传的、昆虫和其他一些无脊椎动物的细胞内细菌共生体。在这里，我们研究了两种不同菌株（在一个大的染色体倒位上有所不同）对雌性昆虫中基因差异表达的影响。我们发现，与未感染的苍蝇相比，感染的苍蝇的转录组有显著变化，与感染 wMelCS 菌株的苍蝇相比，感染 wMelPlus 菌株的苍蝇的转录组也有显著变化。我们将来自两个两两比较的差异表达基因（DEGs），“未感染-感染 wMelPlus”和“未感染-感染 wMelCS”，链接到两个基因网络中，其中指定了以下功能组：“蛋白水解”、“碳水化合物运输和代谢”、“氧化还原过程”、“胚胎发生”、“跨膜运输”、“应激反应”和“碱性磷酸酶”。我们的数据强调了不同菌株感染之间的相似性和差异：与感染 wMelCS 相比，感染 wMelPlus 导致上调的 DEGs 数量增加了一倍以上，而下调的 DEGs 数量减少了一半。因此，我们证明了在宿主基因差异表达方面做出了重大贡献，并且细菌基因型在确定这种贡献的特征方面起着至关重要的作用。
