Kartamihardja Achmad Adhipatria Perayabangsa, Ariyani Winda, Hanaoka Hirofumi, Taketomi-Takahashi Ayako, Koibuchi Noriyuki, Tsushima Yoshito
Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan.
Department of Nuclear Medicine and Molecular Imaging, Universitas Padjajaran, Bandung 40161, Indonesia.
Diagnostics (Basel). 2021 Dec 8;11(12):2310. doi: 10.3390/diagnostics11122310.
Gadolinium deposition in the brain has been observed in areas rich in iron, such as the dentate nucleus of the cerebellum. We investigated the role of Fe in the effect of gadolinium-based contrast agents (GBCA) on thyroid hormone-mediated Purkinje cell dendritogenesis in a cerebellar primary culture. The study comprises the control group, Fe group, GBCA groups (gadopentetate group or gadobutrol group), and GBCA+Fe groups. Immunocytochemistry was performed with an anti-calbindin-28K (anti-CaBP28k) antibody, and the nucleus was stained with 4',6-diamidino-2-phenylindole (DAPI). The number of Purkinje cells and their arborization were evaluated with an analysis of variance with a post-hoc test. The number of Purkinje cells was similar to the control groups among all treated groups. There were no significant differences in dendrite arborization between the Fe group and the control groups. The dendrite arborization was augmented in the gadopentetate and the gadobutrol groups when compared to the control group ( < 0.01, respectively). Fe significantly increased the effect of gadopentetate on dendrite arborization ( < 0.01) but did not increase the effect of gadobutrol. These findings suggested that the chelate thermodynamic stability and Fe may play important roles in attenuating the effect of GBCAs on the thyroid hormone-mediated dendritogenesis of Purkinje cells in in vitro settings.
已在富含铁的脑区（如小脑齿状核）观察到钆沉积。我们在小脑原代培养物中研究了铁在钆基造影剂（GBCA）对甲状腺激素介导的浦肯野细胞树突形成作用中的角色。该研究包括对照组、铁组、GBCA组（钆喷酸组或钆布醇组）以及GBCA+铁组。用抗钙结合蛋白-28K（抗CaBP28k）抗体进行免疫细胞化学检测，并用4',6-二脒基-2-苯基吲哚（DAPI）对细胞核进行染色。采用方差分析及事后检验评估浦肯野细胞的数量及其树突分支情况。所有处理组中浦肯野细胞的数量与对照组相似。铁组与对照组之间在树突分支方面无显著差异。与对照组相比，钆喷酸组和钆布醇组的树突分支增加（分别为P<0.01）。铁显著增强了钆喷酸对树突分支的作用（P<0.01），但未增强钆布醇的作用。这些发现表明，螯合物的热力学稳定性和铁可能在体外环境中减弱GBCA对甲状腺激素介导的浦肯野细胞树突形成的作用方面发挥重要作用。
