Chen Rong-Fu, Liu Keng-Fan, Lee Su-Shin, Huang Shu-Hung, Wu Yi-Chia, Lin Yun-Nan, Wang Chun-Ting, Kuo Yur-Ren
Department of Surgery, Division of Plastic Surgery, Kaohsiung Medical University Hospital, Kaohsiung 80756, Taiwan.
Regenerative Medicine and Cell Therapy Research Center, Department of Biotechnology, Faculty of Medicine, College of Medicine, College of Life Science, Kaohsiung Medical University, Kaohsiung 80756, Taiwan.
Biomedicines. 2021 Dec 15;9(12):1922. doi: 10.3390/biomedicines9121922.
Far-infrared ray (FIR) therapy has been applied in the tissue regeneration field. Studies have revealed that FIR could enhance wound healing. However, the biological effects of FIR on diabetic wounds remain unclear. Our study aims to investigate whether FIR could accelerate diabetic wound healing and analyze the biomechanisms. A dorsal skin defect (area, 6 × 5 cm) in a streptozotocin (STZ)-induced diabetes rodent model was designed. Thirty-two male Wistar rats were divided into 4 groups ( = 8 each subgroup). Group 1 consisted of sham, non-diabetic control; group 2, diabetic control without treatment; group 3, diabetic rats received 20 min FIR (FIR-20, 20 min per session, triplicate/weekly for 4 weeks) and group 4, diabetic rats received 40 min FIR (FIR-40, 40 min per session, triplicate in one week for 4 weeks). The wound healing was assessed clinically. Skin blood flow was measured by laser Doppler. The vascular endothelial growth factor (VEGF), 8-hydroxy-2-deoxyguanosine (8-OHdG), eNOS, and Ki-67, were analyzed with immunohistochemical (IHC) staining. Laser Doppler flowmetry analysis of the blood flow of wounding area revealed the blood flow was higher in diabetic rats who received 40 min FIR (FIR-40) as compared to that in FIR-20 group. The wounding area was significantly reduced in the FIR-40 group than in the diabetic control groups. Histological findings of peri-wounding tissue revealed a significant increase in the neo-vessels in the FIR-treated groups as compared to the controls. IHC staining of periwounding biopsy tissue showed significant increases in angiogenesis expressions (VEGF, eNOS, and EGF), cell proliferation (Ki-67), and suppressed inflammatory response and oxygen radicles (CD45, 8-OHdG) expressions in the FIR-treated groups as compared to that in controls. Treatment with the optimal dosage of FIR significantly facilitated diabetic wound healing and associated with suppressed pro-inflammatory response and increased neovascularization and tissue regeneration.
远红外线（FIR）疗法已应用于组织再生领域。研究表明，FIR可促进伤口愈合。然而，FIR对糖尿病伤口的生物学效应仍不清楚。我们的研究旨在探讨FIR是否能加速糖尿病伤口愈合并分析其生物机制。在链脲佐菌素（STZ）诱导的糖尿病啮齿动物模型中设计了背部皮肤缺损（面积为6×5厘米）。32只雄性Wistar大鼠分为4组（每组8只）。第1组为假手术非糖尿病对照组；第2组为未治疗的糖尿病对照组；第3组为接受20分钟FIR治疗的糖尿病大鼠（FIR - 20，每次治疗20分钟，每周3次，共4周）；第4组为接受40分钟FIR治疗的糖尿病大鼠（FIR - 40，每次治疗40分钟，每周1次，共4周）。临床评估伤口愈合情况。用激光多普勒仪测量皮肤血流量。用免疫组织化学（IHC）染色分析血管内皮生长因子（VEGF）、8 - 羟基 - 2 - 脱氧鸟苷（8 - OHdG）、内皮型一氧化氮合酶（eNOS）和Ki - 67。激光多普勒血流仪对伤口区域血流的分析显示，接受40分钟FIR治疗的糖尿病大鼠（FIR - 40）的血流量高于FIR - 20组。FIR - 40组的伤口面积比糖尿病对照组明显减小。伤口周围组织的组织学检查结果显示，与对照组相比，FIR治疗组的新血管显著增加。伤口周围活检组织的IHC染色显示，与对照组相比，FIR治疗组的血管生成表达（VEGF、eNOS和表皮生长因子（EGF））、细胞增殖（Ki - 67）显著增加，炎症反应和氧自由基（CD45、8 - OHdG）表达受到抑制。最佳剂量的FIR治疗显著促进了糖尿病伤口愈合，并与炎症反应抑制、新血管形成增加和组织再生有关。
