透明质酸-聚维酮碘复合物促进链脲佐菌素诱导的糖尿病啮齿动物模型中的糖尿病伤口愈合。
Hyaluronic Acid-Povidone-Iodine Compound Facilitates Diabetic Wound Healing in a Streptozotocin-Induced Diabetes Rodent Model.
机构信息
From the Division of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital; the Department of Healthcare Materials, Material and Chemical Research Laboratories, Industrial Technology Research Institute; the Department of Orthopaedics, Kaohsiung Chang Gung Memorial Hospital; the Faculty of Medicine, College of Medicine, Orthopaedic Research Center, Kaohsiung Medical University; and the Department of Biological Sciences, National Sun Yat-sen University.
出版信息
Plast Reconstr Surg. 2019 May;143(5):1371-1382. doi: 10.1097/PRS.0000000000005504.
BACKGROUND
This study investigated whether a hyaluronic acid-povidone-iodine compound can enhance diabetic wound healing.
METHODS
A dorsal skin defect (6 × 5 cm) in a streptozotocin-induced diabetes rodent model was used. Seventy male Wistar rats were divided into seven groups: I, normal control; II, diabetic control, no treatment; III, diabetic rats, lower molecular weight (100 kDa) hyaluronic acid; IV, rats, higher molecular weight (1000 kDa) hyaluronic acid; V, rats, 0.1% povidone-iodine; VI, rats, lower molecular weight hyaluronic acid plus povidone-iodine; and VII, rats, higher molecular weight hyaluronic acid plus povidone-iodine. Histologic examination was performed with hematoxylin and eosin staining. CD45, Ki-67, prolyl 4-hydroxylase, and vascular endothelial growth factor were evaluated with immunohistochemical staining.
RESULTS
Compared with the control, higher molecular weight hyaluronic acid plus povidone-iodine-treated rats had significantly reduced wound area (p < 0.001). Higher molecular weight hyaluronic acid plus povidone-iodine increased wound healing time when compared with higher molecular weight hyaluronic acid, povidone-iodine, or lower molecular weight hyaluronic acid plus povidone-iodine. Histology revealed significantly increased neovessels and suppressed inflammatory response in the higher molecular weight hyaluronic acid plus povidone-iodine group when compared with the control group. Immunohistochemical staining revealed significantly increased Ki67, prolyl 4-hydroxylase, and vascular endothelial growth factor expression, and suppressed CD45 expression in the higher molecular weight hyaluronic acid plus povidone-iodine group when compared with the other groups.
CONCLUSION
Higher molecular weight hyaluronic acid plus povidone-iodine complex dressing significantly facilitated diabetic wound healing via increasing neovascularization and tissue regeneration and suppressing a proinflammatory response.
背景
本研究旨在探讨透明质酸-聚维酮碘复合物是否能促进糖尿病创面愈合。
方法
采用链脲佐菌素诱导的糖尿病大鼠模型制作背部皮肤缺损（6×5cm）。70 只雄性 Wistar 大鼠分为 7 组：I 组，正常对照组；II 组，糖尿病对照组，未治疗；III 组，低分子质量（100kDa）透明质酸组；IV 组，高分子质量（1000kDa）透明质酸组；V 组，0.1%聚维酮碘组；VI 组，低分子质量透明质酸加聚维酮碘组；VII 组，高分子质量透明质酸加聚维酮碘组。苏木精-伊红染色行组织学检查。免疫组织化学染色检测 CD45、Ki-67、脯氨酸 4-羟化酶和血管内皮生长因子。
结果
与对照组相比，高分子质量透明质酸加聚维酮碘治疗组的创面面积明显减小（p<0.001）。与高分子质量透明质酸、聚维酮碘或低分子质量透明质酸加聚维酮碘相比，高分子质量透明质酸加聚维酮碘增加了创面愈合时间。与对照组相比，高分子质量透明质酸加聚维酮碘组的新生血管明显增加，炎症反应受到抑制。免疫组织化学染色显示，与其他组相比，高分子质量透明质酸加聚维酮碘组 Ki67、脯氨酸 4-羟化酶和血管内皮生长因子表达明显增加，CD45 表达受到抑制。
结论
高分子质量透明质酸加聚维酮碘复合敷料通过增加新生血管和组织再生，抑制促炎反应，显著促进糖尿病创面愈合。
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