Rosés Carles, Nieto Juan Antonio, Viadel Blanca, Gallego Elisa, Romo-Hualde Ana, Streitenberger Sergio, Milagro Fermín I, Barceló Anna
Servei de Genòmica, Universitat Autònoma de Barcelona, Bellaterra, 08193 Cerdanyola del Vallés, Spain.
Ainia, In Vitro Digestion Assays, Parque Tecnológico de Valencia, 46980 Paterna, Spain.
Foods. 2021 Dec 5;10(12):3020. doi: 10.3390/foods10123020.
The gut microbiota plays a key role in gastrointestinal immune and metabolic functions and is influenced by dietary composition. An in vitro protocol simulating the physiological conditions of the digestive system helps to study the effects of foods/biocompounds on gut microbiome and metabolome. The Dynamic-Colonic Gastrointestinal Digester consists of five interconnected compartments, double jacket vessels that simulate the physiological conditions of the stomach, the small intestine and the three colonic sections, which are the ascending colon, transverse colon and descending colon. Human faeces are required to reproduce the conditions and culture medium of the human colon, allowing the growth of the intestinal microbiota. After a stabilization period of 12 days, a food/biocompound can be introduced to study its modulatory effects during the next 14 days (treatment period). At the end of the stabilization and treatment period, samples taken from the colon compartments are analysed. The 16S rRNA gene analysis reveals the microbiota composition. The untargeted metabolomics analysis gives more than 10,000 features (metabolites/compounds). The present protocol allows in vitro testing of the modulatory effects of foods or biocompounds on gut microbiota composition and metabolic activity.
肠道微生物群在胃肠道免疫和代谢功能中起关键作用，并受饮食组成的影响。一种模拟消化系统生理条件的体外实验方案有助于研究食物/生物活性化合物对肠道微生物组和代谢组的影响。动态结肠胃肠消化器由五个相互连接的隔室组成，即双层夹套容器，可模拟胃、小肠以及升结肠、横结肠和降结肠这三个结肠段的生理条件。需要使用人类粪便来重现人类结肠的条件和培养基，以促进肠道微生物群的生长。在12天的稳定期后，可以引入一种食物/生物活性化合物，以研究其在接下来14天（治疗期）内的调节作用。在稳定期和治疗期结束时，对从结肠隔室采集的样本进行分析。16S rRNA基因分析可揭示微生物群的组成。非靶向代谢组学分析可给出10000多个特征（代谢物/化合物）。本实验方案允许对食物或生物活性化合物对肠道微生物群组成和代谢活性的调节作用进行体外测试。
