Shojania A M, Orr K
Clin Immunol Immunopathol. 1987 May;43(2):223-8. doi: 10.1016/0090-1229(87)90130-9.
Our observation that the effect of a patient's autoantibody with a potent antithrombin activity could be inhibited by toluidine blue and methylene blue led us to investigate the effect of these dyes in several immunochemical reactions in vitro. Both dyes reduced the titer or prevented the detection of IgG-specific single- and double-stranded DNA-binding antibodies and rheumatoid factors but did not reduce the titer of antinuclear factor and had no effect on detection of microsomal and thyroglobulin antibodies. These dyes did not aggregate or precipitate the immunoglobulins and their inhibitory effect in serum could be removed by dialysis of serum. The possible mechanism of action of these dyes in immunochemical reactions is discussed.
我们观察到，具有强效抗凝血酶活性的患者自身抗体的作用可被甲苯胺蓝和亚甲蓝抑制，这促使我们研究这些染料在体外几种免疫化学反应中的作用。两种染料均降低了IgG特异性单链和双链DNA结合抗体以及类风湿因子的效价，或阻止了其检测，但未降低抗核因子的效价，且对微粒体抗体和甲状腺球蛋白抗体的检测没有影响。这些染料不会使免疫球蛋白聚集或沉淀，血清中的抑制作用可通过血清透析去除。本文讨论了这些染料在免疫化学反应中可能的作用机制。
