Araújo Patrícia V, Teixeira Karina I R, Lanza Lincoln D, Cortes Maria E, Poletto Luiz T A
Departamento de Odontologia Restauradora da Faculdade de Odontologia da Universidade Federal de Minas Gerais, Brazil.
Acta Odontol Latinoam. 2009;22(2):93-7.
The purpose of this in vitro study was to evaluate the antimicrobial effect of photodynamic therapy on Streptococcus mutans (A TCC 25175) suspensions, using a red laser for one minute in combination with toluidine blue O (TBO) or methylene blue (MB). Both photosensitizers were used in three concentrations (25, 10 and 5 mg/L). The activity ofphotosensitizers and laser irradiation were tested separately on the bacteria, as well as the irradiation of this light source in the presence of the TBO or MB. These groups were compared to a control group, in which the microorganism did not receive any treatment. The activity of both TBO and MB or laser irradiation, alone, were not able to reduce the number of S. mutans. In the groups of lethal photosensitization, a bacterial reduction of 70% for TBO and 73% for MB was observed when these photosensitizers were used at 25 mg/L and a reduction of 48% was observed for MB at 5mg/L. In other concentrations there were no significant differences in comparison to the control group. Both the TBO and the MB at 25 mg/L associated with a red laser had an excellent potential for use in PDT in lethal sensitization of S. mutans.
本体外研究的目的是评估光动力疗法对变形链球菌（ATCC 25175）悬液的抗菌效果，使用红色激光照射一分钟，并联合使用甲苯胺蓝O（TBO）或亚甲蓝（MB）。两种光敏剂均采用三种浓度（25、10和5mg/L）。分别测试了光敏剂和激光照射对细菌的作用，以及在TBO或MB存在下该光源的照射。将这些组与未接受任何处理的对照组进行比较。单独的TBO和MB或激光照射均不能减少变形链球菌的数量。在致死性光敏化组中，当使用25mg/L的这些光敏剂时，观察到TBO使细菌减少70%，MB使细菌减少73%；当使用5mg/L的MB时，观察到细菌减少48%。在其他浓度下，与对照组相比无显著差异。25mg/L的TBO和MB与红色激光联合使用在变形链球菌致死性光敏化的光动力疗法中具有极好的应用潜力。
