Ma Zhi, Tang Xia, Stanford Kim, Chen Xiaolong, McAllister Tim A, Niu Yan D
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB T1J 4B1, Canada.
Microorganisms. 2021 Dec 3;9(12):2510. doi: 10.3390/microorganisms9122510.
Shiga toxin-producing (STEC) and are important foodborne pathogens capable of forming both single- and multi-species biofilms. In this study, the mono- and dual-species biofilms were formed by STEC O113:H21 and serovar Choleraesuis 10708 on stainless steel in the presence of beef juice over 5 d at 22 °C. The dual-species biofilm mass was substantially ( < 0.05) greater than that produced by STEC O113:H21 or Choleraesuis 10708 alone. However, numbers (CFU/mL) of . Choleraesuis 10708 or STEC O113:H21 cells in the dual-species biofilm were ( < 0.05) lower than their respective counts in single-species biofilms. In multi-species biofilms, the sensitivity of . Choleraesuis 10708 to the antimicrobial peptide WK2 was reduced, but it was increased for STEC O113:H21. Visualization of the temporal and spatial development of dual-species biofilms using florescent protein labeling confirmed that WK2 reduced cell numbers within biofilms. Collectively, our results highlight the potential risk of cross-contamination by multi-species biofilms to food safety and suggest that WK2 may be developed as a novel antimicrobial or sanitizer for the control of biofilms on stainless steel.
产志贺毒素大肠杆菌（STEC）是重要的食源性病原体，能够形成单物种和多物种生物膜。在本研究中，STEC O113:H21和猪霍乱血清型10708在22℃下于牛肉汁存在的条件下在不锈钢表面形成单物种和双物种生物膜，持续5天。双物种生物膜量显著（P<0.05）大于单独由STEC O113:H21或猪霍乱10708产生的生物膜量。然而，双物种生物膜中猪霍乱10708或STEC O113:H21细胞的数量（CFU/mL）显著（P<0.05）低于它们在单物种生物膜中的各自数量。在多物种生物膜中，猪霍乱10708对抗菌肽WK2的敏感性降低，但STEC O113:H21的敏感性增加。使用荧光蛋白标记对双物种生物膜的时空发育进行可视化证实，WK2减少了生物膜内的细胞数量。总体而言，我们的结果突出了多物种生物膜对食品安全造成交叉污染的潜在风险，并表明WK2可能被开发为一种新型抗菌剂或消毒剂，用于控制不锈钢表面的生物膜。
