Pham Van Hong Thi, Kim Jaisoo, Chang Soonwoong, Chung Woojin
Department of Environmental Energy Engineering, Graduate School of Kyonggi University, Suwon 16227, Korea.
Department of Life Science, College of Natural Science of Kyonggi University, Suwon 16227, Korea.
Microorganisms. 2021 Dec 15;9(12):2590. doi: 10.3390/microorganisms9122590.
Compared to lipases from plants or animals, microbial lipases play a vital role in different industrial applications and biotechnological perspectives due to their high stability and cost-effectiveness. Therefore, numerous lipase producers have been investigated in a variety of environments in the presence of lipidic carbon and organic nitrogen sources. As a step in the development of cultivating the unculturable functional bacteria in this study, the forest soil collected from the surrounding plant roots was used to create an artificially contaminated environment for lipase-producing bacterial isolation. The ten strongest active bacterial strains were tested in an enzyme assay supplemented with metal ions such as Ca, Zn, Cu, Fe, Mg, K, Co, Mn, and Sn to determine bacterial tolerance and the effect of these metal ions on enzyme activity. Lipolytic bacteria in this study tended to grow and achieved a high lipase activity at temperatures of 35-40 °C and at pH 6-7, reaching a peak of 480 U/mL and 420 U/mL produced by PL33 and PL35, respectively. These potential lipase-producing bacteria are excellent candidates for large-scale applications in the future.
与植物或动物来源的脂肪酶相比，微生物脂肪酶因其高稳定性和成本效益，在不同的工业应用和生物技术领域发挥着至关重要的作用。因此，众多脂肪酶产生菌已在含有脂质碳源和有机氮源的各种环境中得到研究。作为本研究中培养不可培养功能细菌的一个步骤，从周围植物根部采集的森林土壤被用于创建一个用于分离产脂肪酶细菌的人工污染环境。在补充了钙、锌、铜、铁、镁、钾、钴、锰和锡等金属离子的酶活性测定中，对十种活性最强的细菌菌株进行了测试，以确定细菌的耐受性以及这些金属离子对酶活性的影响。本研究中的解脂细菌倾向于在35 - 40°C的温度和pH值6 - 7的条件下生长并达到高脂肪酶活性，PL33和PL35分别产生的酶活峰值为480 U/mL和420 U/mL。这些潜在的产脂肪酶细菌是未来大规模应用的优秀候选菌株。
