Bopegamage Shubhada, Berakova Katarina, Gomocak Pavol, Baksova Renata, Galama Jochem, Hyoty Heikki, Tauriainen Sisko
Enterovirus Laboratory, Institute of Microbiology, Faculty of Medicine, Slovak Medical University, Limbova 12, 833 03 Bratislava, Slovakia.
Martinske Biopticke Centrum s.r.o., V. Spanyola 47A Street, 010 01 Zilina, Slovakia.
Microorganisms. 2021 Dec 16;9(12):2600. doi: 10.3390/microorganisms9122600.
Enterovirus (EV) infections are associated with a broad range of diseases. Since the first experimental infection of primates with poliovirus (PV), tonsils and the Peyer's patches (PPs) have been believed to be the primary replication sites of EVs. Our aim was to localize different viral markers in the small intestines (SI) of coxsackievirus B (CVB) orally and intraperitoneally (i.p.) infected mice.
Transverse sections of SIs of both infected and control male outbred mice were collected at different intervals post-infection (p.i) and analyzed for presence of interferon-alpha (IFN-α) and viral protein VP1 by immunohistochemistry and in situ hybridization (ISH). Fluorescent marker, eGFP, was identified in cryosections of mice infected with eGFP-CVB3.
In the infected SIs, we observed enlarged germinating centers (GCs) in the PPs; IFN-α was detected in the PPs and mucosal layer of the SIs. However, VP1, viral RNA and the eGFP were absent in the GCs of PPs at all stages of infection irrespective of the virus strains used.
Virus was present in the epithelial cells but not in GCs of the PPs of the murine SIs. Our results do not support the hypothesis of EV replication in the PP especially in the GCs.
肠道病毒（EV）感染与多种疾病相关。自从首次用脊髓灰质炎病毒（PV）对灵长类动物进行实验性感染以来，扁桃体和派尔集合淋巴结（PPs）一直被认为是EV的主要复制部位。我们的目的是在经口和腹腔内（i.p.）感染柯萨奇病毒B（CVB）的小鼠小肠（SI）中定位不同的病毒标志物。
在感染后（p.i）的不同时间间隔收集感染组和对照组雄性远交系小鼠的SI横切片，通过免疫组织化学和原位杂交（ISH）分析干扰素-α（IFN-α）和病毒蛋白VP1的存在情况。在感染eGFP-CVB3的小鼠冰冻切片中鉴定荧光标志物eGFP。
在感染的SI中，我们观察到PPs中发芽中心（GCs）增大；在PPs和SI的粘膜层中检测到IFN-α。然而，无论使用何种病毒株，在感染的各个阶段，PPs的GCs中均未检测到VP1、病毒RNA和eGFP。
病毒存在于小鼠SI的上皮细胞中，但不存在于PPs的GCs中。我们的结果不支持EV在PPs尤其是GCs中复制的假说。
