Fridman Sophie, Tsairidou Smaragda, Jayasuriya Nilantha, Sobolewska Halina, Hamilton Alastair, Lobos Carlos, Houston Ross D, Rodger Hamish, Bron James, Herath Tharangani
Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK.
The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh EH25 9RG, UK.
Microorganisms. 2021 Dec 16;9(12):2605. doi: 10.3390/microorganisms9122605.
Gill disorders have become more prevalent and widespread in finfish aquaculture in recent years. Their aetiology is often considered to be multifactorial. Effective diagnosis, control and prevention are hindered by the lack of standardised methodologies to characterise the aetiological agents, which produce an array of clinical and pathological presentations. The aim of this study was to define a novel gross pathological scoring system suitable for field-based macroscopic assessment of complex or multifactorial gill disease in farmed Atlantic salmon, using samples derived from a gill disease outbreak in Chile. Clinical assessment of gross gill morphology was performed, and gill samples were collected for qPCR and histology. A novel total gill scoring system was developed, which assesses gross pathological changes combining both the presumptive or healed amoebic gill disease (AGD) and the presence of other types of gill lesions. This scoring system offers a standardised approach to characterise the severe proliferative pathologies in affected gills. This total gill scoring system can substantially contribute to the development of robust mitigation strategies and could be used as an indicator trait for incorporating resistance to multifactorial gill disease into breeding goals.
近年来，鳃病在硬骨鱼类养殖中变得更加普遍和广泛。其病因通常被认为是多因素的。由于缺乏标准化的方法来鉴定病原体，而这些病原体可导致一系列临床和病理表现，因此有效诊断、控制和预防受到阻碍。本研究的目的是使用来自智利一次鳃病暴发的样本，定义一种适用于现场宏观评估养殖大西洋鲑复杂或多因素鳃病的新型大体病理评分系统。对鳃的大体形态进行了临床评估，并采集鳃样本用于定量聚合酶链反应（qPCR）和组织学检查。开发了一种新型的全鳃评分系统，该系统结合假定的或已愈合的阿米巴鳃病（AGD）以及其他类型鳃病变的存在来评估大体病理变化。该评分系统提供了一种标准化方法来描述受影响鳃中的严重增殖性病变。这种全鳃评分系统可极大地促进稳健缓解策略的制定，并可作为将对多因素鳃病的抗性纳入育种目标的指标性状。
