Sandström G, Tärnvik A, Wolf-Watz H
J Clin Microbiol. 1987 Apr;25(4):641-4. doi: 10.1128/jcm.25.4.641-644.1987.
Membranes from a capsule-deficient mutant of the live-vaccine strain of Francisella tularensis (F. tularensis LVS) were treated with N-lauroyl sarcosinate (Sarkosyl; CIBA-GEIGY Corp., Summit, N.J.). When the Sarkosyl-insoluble fraction was heated in the presence of sodium dodecyl sulfate and mercaptoethanol and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, several polypeptides were distinguished. Four major polypeptides were eluted from the gel, each of which stimulated lymphocytes from tularemia-vaccinated individuals but not from nonvaccinated individuals. The stimulation occurred mainly in T lymphocytes. Radioactive labeling of surface proteins of the capsule-deficient bacteria indicated that at least two of the four polypeptides originated from outer membrane proteins. The results suggest that several membrane proteins of F. tularensis LVS induce a specific T-lymphocyte response.
用N-月桂酰肌氨酸钠（十二烷基肌氨酸钠；CIBA - GEIGY公司，新泽西州萨米特）处理土拉热弗朗西斯菌（F. tularensis LVS）活疫苗株的荚膜缺陷突变体的膜。当十二烷基肌氨酸钠不溶部分在十二烷基硫酸钠和巯基乙醇存在下加热并进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳时，可区分出几种多肽。从凝胶中洗脱了四种主要多肽，每种多肽都能刺激来自接种土拉热疫苗个体的淋巴细胞，但不能刺激未接种个体的淋巴细胞。这种刺激主要发生在T淋巴细胞中。对荚膜缺陷细菌表面蛋白的放射性标记表明，这四种多肽中至少有两种源自外膜蛋白。结果表明，土拉热弗朗西斯菌LVS的几种膜蛋白可诱导特异性T淋巴细胞反应。
